P30A: Biomaterials Core (6 of 10)

生物材料和组织工程在生殖生物学中的应用为生殖生物学的发展提供了一个有利的条件 作为肿瘤生育联盟发展基础的技术。目前正在开发一个核心， 这项技术的翻译。这个拟议的核心有两个主要任务：i）研究，以进一步发展这一 使技术能够确定冷冻保存和成熟灵长类动物卵泡的条件，ii）服务于 向卫星地点提供成熟程序的材料和培训。先前的工作已经 证明了藻酸盐水凝胶通过维持毛囊的生长来模仿体内环境， 结构，同时呈现出可扩散的、不溶性的和机械信号的组合，这些信号结合联合收割机， 影响卵泡的发育。卵巢卵泡可以在体外成熟以产生高质量的卵母细胞 可以受精并支持小鼠的活产。本项目的研究目标是 由于需要开发水凝胶，i）支持大的灵长类动物卵泡的生长， 与小鼠卵泡不同的要求，ii）维持卵泡结构并促进在 冷冻保存，和iii）促进移植后移植卵泡的植入和存活。 因此，该核心的活动侧重于四个具体目标。目标1：为生物技术领域提供生物材料支持 肿瘤生育联合体（R01A、R01B、R01C、P30A）。目标2：确定生物材料特性和培养 条件，将最大限度地提高灵长类卵泡生长，这将被翻译为R01B和R01C。目标3： 开发新型生物材料，可用于最大限度地减少组织冷冻损伤，以转化为R01A和P30B。 目标4：设计药物释放水凝胶以优化皮质带移植（R 01 B）。一个令人兴奋的团队， 研究人员已经聚集在一起，将新技术转化为重要的临床应用。 问题.重要的是，可以立即产生可受精的卵母细胞和健康的胚胎。 鉴于现有的体外受精基础设施，

The application of biomaterials and tissue engineering to reproductive biology provides an enabling technology underlying the development of the Oncofertility Consortium. A core is being developed for the translation of this technology.This proposed core has two major missions: i) research to further develop this enabling technology identify conditions for cryopreserving and maturing primate ovarian follicles, ii) service to provide materials and training to satellite locations for the maturation procedures. Previous work has demonstrated that the hydrogel alginate phenocopies the in vivo environment by maintaining follicular architecture while presenting a combination of diffusible, insoluble, and mechanical signals that combine to influence the development of the follicle. Ovarian follicles can be matured in vitro to yield high quality oocytes that can be fertilized and support live births with mice. The research objectives of this project are motivated by the need to develop hydrogels that i) support the growth of the large primate follicles that may have different requirements from mouse follicles, ii) maintain the follicle architecture and facilitate handling during cryopreservation, and iii) promote engraftment and survival of transplanted follicles following transplantation. The activities of this core are thus focused on four specific goals. Goal 1: Provide biomaterial support to the oncofertility consortium (R01A, R01B, R01C, P30A). Goal 2: Identify biomaterial properties and culture conditions that will maximize primate follicle growth, which will be translated to R01B and R01C. Goal 3: Develop novel biomaterials that can be used to minimize tissue cryoinjury for translation to R01A and P30B. Goal 4: Engineer drug-releasing hydrogels to optimize cortical strip transplants (R01B). An exciting team of investigators has been assembled towards translating novel technologies towards a significant clinical problem. Importantly, the generation of fertilizable oocytes and healthy embryos can immediately be translated clinically given the existing infrastructure for in vitro fertilization.