T LYMPHOCYTE, T CELL RECEPTOR, CELLULAR IMMUNITY, IMMUNOTHERAPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究的目的是研究持续的急性和慢性感染以及对肿瘤的暴露如何影响原始旁观者T细胞数量。 在报告期间，我们继续我们的研究，分析急性和慢性感染期间NAéve T细胞的状态、动力学和稳定性，并确定在持续感染或持续肿瘤期间慢性抗原暴露对旁观者NAéve T细胞安装免疫反应的能力的影响。我们还致力于确定在慢性感染或肿瘤模型中引入治疗性细胞因子对旁观者NAVE T细胞的影响。 采用TCR转基因模型，通过过继转移，将OVA TCR NA？ve T细胞暴露于急性LCMV感染或Lewis肺癌移植瘤模型中，回收OVA NA？ve细胞，检测其对OVA感染的应答能力。 我们将继续努力开发治疗时机的线索，这些线索将导致针对慢性病和免疫抑制(如艾滋病毒感染)中的NA？T细胞活性的治疗开发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to examine how ongoing acute and chronic infections and exposure to tumors affect na¿ve bystander T cell populations. During the reporting period, we continued our studies analyzing the status, kinetics, and stability of na¿ve T cells during acute and chronic infections and to determine how chronic antigen exposure during persistent infections or persisting tumors has on the ability of bystander na¿ve T cells to mount immune responses. We also worked to determine the effect of introducing therapeutic cytokines during a chronic infection or tumor model has on bystander na¿ve T cells. Using a TCR transgenic model we exposed, by adoptive transfer, OVA TCR na¿ve T cells to acute LCMV infection or Lewis Lung Carcinoma tumor transplant model, recovered OVA na¿ve cells then determine their ability to respond to OVA infection. We will continue to work to develop clues in the timing of therapies that will lead to therapeutic developments for targeting na¿ve T cell viability during chronic diseases and immune suppression, for example HIV infection.