REGIONAL VARIATION IN D1 SIGNALING PROTEIN LOCALIZATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们研究了多巴胺如何作用于D1家族多巴胺受体(D1R；D1和D5)，影响与精神分裂症、创伤后应激障碍和药物滥用相关的大脑区域的电路。这些区域包括前额叶皮质(PFC)、杏仁核和伏隔核(NAC)。我们在(PFC)中完成了对D1R的检查，并发表了结果。我们进一步研究了D1R在前额叶中间神经元亚型中的分布。我们在中间神经元亚群中发现了不同的受体定位模式，并提出了一个详细的模型，解释了多巴胺刺激D1家族受体如何控制锥体细胞。这部作品的手稿正在印刷中。 我们完成了对猴子和大鼠杏仁核D1R的研究，杏仁核是一个与恐惧和焦虑症以及药物成瘾有关的大脑区域。我们发现D1和D5之间以及PFC和杏仁核之间的分布存在差异。我们在大鼠和猴子杏仁核中发现了相似的分布模式，与先前在大鼠中的报道相比，猴子的细胞反应相似。正在准备提交这些结果。此外，我们研究了灵长类杏仁核的中间神经元亚型，发现猴子的PV中间神经元与大鼠的类似。这部作品正在付印中。 我们对D1R在NAC中的研究已接近完成，我们正在表征D1R信号转导途径的成分：DARPP-32和抑制物-1的分布。我们已经完成了PFC的分析，并正在准备提交手稿。我们正在努力完成对杏仁核和NAC的分析。我们对LFC蛋白的研究开创了一种新的定位分析方法，并已发表。最后，我们发表了一项关于杏仁核不同部位mGluR2/3的研究，并证实了我们的分析显示了显著的功能差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We study how dopamine acting at D1 family dopamine receptors (D1R; D1 and D5) affects the circuitry of brain regions relevant to schizophrenia, PTSD and substance abuse. These regions include prefrontal cortex (PFC), amygdala and nucleus accumbens (NAc). We completed our examination of D1R in (PFC) and published the results. We have further examined the distribution of D1R in prefrontal interneuron subtypes. We have found distinct patterns of localization of receptors in subpopulations of interneurons and proposed a detailed model for how dopamine stimulation of D1 family receptors controls pyramidal cell. A manuscript of this work is in press. We completed our study of D1R in monkey and rat amygdala, a brain region implicated in fear and anxiety disorders, as well as drug addiction. We found differences in the distribution between D1 and D5 and between PFC and amygdala. We found similar patterns of distribution between rat and monkey amygdala and similar cellular responses in monkey compared to previous reports in rat. These results are being prepared for submission. In addition, we examined the interneuron subtypes of the primate amygdala and found that PV interneurons in monkey are similar to those seen in rat. This work is in press. Our studies of D1R in NAc are nearly complete, and we are characterizing the distribution of components of the D1R signal transduction pathway: DARPP-32 and inhibitor-1. We have completed our analysis in PFC and are preparing a manuscript for submission. We are working to complete our analysis in the amygdala and NAc. Our study of the protein Lfc has pioneered a new localization assay and has been published. Finally, we published a study of mGluR2/3 in different parts of the amygdala and confirmed that our analyses reveal functionally significant differences.