In Vivo Analysis of Adenosine Ribonucleosides by Microdialysis and Capillary Elec

描述（由申请人提供）：该项目的长期成果将是一种高度敏感，快速，强大的分析技术，可广泛用于神经科学家和神经学家监测大脑中腺苷和相关分子的实时变化。这项研究将允许进一步研究这些化合物在中风或癫痫引起的神经元损伤中，在神经退行性疾病如帕金森病、亨廷顿病或阿尔茨海默病以及精神疾病如精神分裂症和成瘾行为中所起的作用。这种分析技术的基础是腺苷衍生物在含有-环糊精的缓冲液中的显著荧光增强。该衍生物的荧光增强将允许用激光诱导荧光检测毛细管电泳分析生物样品。将对反应动力学和荧光进行优化，然后对该技术进行分析和药理验证。

DESCRIPTION (provided by applicant): The long term outcome of this project will be a highly sensitive, rapid, robust analytical technique that can be broadly employed by neuroscientists and neurologists to monitor real-time changes in adenosine and related molecules in the brain. This research will allow further study of the involvement of these compounds in the neuronal injury induced by stroke or epilepsy, in neurodegenerative disorders such as Parkinson's disease, Huntington's disease or Alzheimer's disease and in psychiatric disorders such as schizophrenia and addictive behaviour. The basis of this analytical technique is the dramatic fluorescence enhancement of an adenosine derivative by a buffer containing -cyclodextrin. The fluorescence enhancement of the derivative will allow analysis of biological samples by capillary electrophoresis with laser-induced fluorescence detection. Reaction kinetics and fluorescence will be optimized, followed by analytical and pharmacological validation of the technique. PUBLIC HEALTH RELEVANCE: The long term outcome of this project will be a highly sensitive, rapid, robust analytical technique that can be broadly employed by neuroscientists and neurologists to monitor real-time changes in Adenosine and related molecules in the brain. This will allow to further study the involvement of these compounds in the neuronal injury induced by stroke or epilepsy, in neurodegenerative disorders such as Parkinson's disease, Huntington's disease or Alzheimer's disease and in psychiatric disorders such as schizophrenia and addictive behavior.