Ohio State Neuroscience Center Core

项目总结(见说明)： 俄亥俄州立大学(OSU)神经科学中心为神经疾病的分析和治疗研究提供支持，重点是动物模型。该中心是来自俄亥俄州立大学和全国儿童医院的43名神经学家(25名NINDS资助)共同努力的成果。这些PIS大多研究神经系统疾病，四个主要焦点领域是运动神经元疾病、神经肌肉疾病、中枢神经系统和三叉神经系统损伤以及脑肿瘤。这些PI横跨俄亥俄州立大学的许多部门、中心和研究所，这些部门、中心和研究所支持基础和翻译神经科学研究，使该中心成为校园神经科学活动的核心。中心的优先事项与俄亥俄州立大学的神经科学签名计划保持一致，并可以利用该计划，这是一项耗资数百万美元的努力，支持神经科学作为校园五个增长领域之一。此外，该中心与新近授予俄亥俄州立大学医学中心的临床和翻译科学奖保持一致。为了支持神经系统疾病模型的开发、表征和分析，该提案支持5个研究核心。核心A(行政)将监督俄亥俄州立大学神经科学中心。核心B(遗传学)将支持转基因/基因敲除小鼠的发展和斑马鱼的使用，重点是传统和新的遗传方法。此外，该核心将支持基因组操纵设施，重点是锌指核酸酶和BAC重组工程技术，以产生新的动物模型。CORE C(CNS/PNS损伤和啮齿动物行为)将提供设备和技术专长，以生成损伤模型并分析与这些模型相关的行为缺陷。CORE D(生理学)将为电生理学和肌肉生理学提供设备和技术援助，以分析这些模型。Core E(成像)将以更低的成本和技术援助支持共焦成像和核磁共振成像。Core F(异种移植)将提供设备和技术援助，以产生脑瘤的小鼠模型。 这些独特的核心将为PI提供任何单一实验室都无法获得的集中设备和技术专业知识，从而大大加强俄亥俄州立大学园区的神经科学研究。

PROJECT SUMMARY (See instructions): The Ohio State University (OSU) Neuroscience Center provides support for studies ainned at analysis and treatment of neurological disorders with an emphasis on animal models. The Center is a collaborative effort of 43 neuroscientists (25 NINDS funded) from OSU and Nationwide Children's Hospital. The majority of these Pis work on aspects of nervous system disorders with four major focus areas being motoneuron disease, neuromuscular disease, CNS and PNS injury, and brain tumors. These Pis span numerous departments, centers, and institutes at OSU that support basic and translational neuroscience research making the Center a nucleus of neuroscience activity on campus. Center priorities align with and can leverage the Neuroscience Signature Program at OSU, a multi-million dollar effort supporting Neuroscience as one of five growth areas on campus. In addition, the Center aligns with the newly granted Clinical and Translational Science Award to the OSU Medical Center. To support the development, characterization and analysis of nervous system disease models, this proposal supports 5 research Cores. Core A (Administrative) will oversee the OSU Neuroscience Center. Core B (Genetics) will support the development of transgenic/knockout mice and the use of zebrafish with an emphasis on traditional and novel genetic approaches. In addition this Core will support a Genome Manipulation Facility focusing on zinc-finger nuclease and BAC recombineering techniques for the generation of new animal models. Core C (CNS/PNS Injury and Rodent Behavior) will provide the equipment and technical expertise to generate injury models and to analyze behavioral deficits associated with these models. Core D (Physiology) will provide equipment and technical assistance for both electrophysiology and muscle physiology to analyze these models. Core E (Imaging) will support both Confocal and MRI imaging with reduced costs and technical assistance. Core F (Xenograft) will provide equipment and technical assistance to generate mouse models of brain tumors. These unique Cores will provide Pis with access to centralized equipment and technical expertise unavailable in any single lab, thus substantially enhancing neuroscience research on the OSU campus.