Mass Spectrometry

质谱学技术，特别是那些需要测量生物样品中稳定的(非放射性)核素浓缩的技术，已经成为研究人类营养和新陈代谢的既定工具。这种方法虽然对技术要求很高，但特别适合于各种病理生理状态下的体内定量研究。Core将全身示踪方法与组织特定的移动PET成像相结合的倡议是一种评估代谢变化的新方法，对肥胖和非肥胖受试者具有重要的适用性。同样，在核心中增加代谢组学和脂类组学成分将进一步扩大NORC-H研究人员可获得的营养和代谢研究的范围。该核心、其董事和联席董事以及其高级技术人员将提供：1.协助设计成功的营养稳定同位素和PET实验，包括示踪剂选择、用于输注的同位素准备，以及关于机构审查委员会批准的咨询。2.生物标志物和代谢物发现、生物标志物和代谢物鉴定和代谢物定量的代谢组学平台。3.高效和公平地获得核心分析仪器、准确的样品分析和质量控制。4.协助开发分析血液、尿液、组织和呼出空气等生物样本中化合物的新方法。5.新的研究人员有机会了解稳定同位素示踪技术、正电子发射计算机断层扫描和代谢组学在代谢/营养研究中的应用。

Mass spectrometry techniques, particularly those requiring the measurement of stable (non-radioactive) nuclide enrichment in biological samples, have become established tools in the study of human nutrition and metabolism. Such methods, though technically demanding, are uniquely suited to quantitative In vivo studies under various pathophysiologic states. The Core's initiative to combine whole body tracer approaches with tissue specific mobile PET imaging is a novel means to evaluate metabolic alterations and has significant applicability to obese and non-obese subjects. Likewise, the addition of a metabolomics and lipidomics component to the Core will further enhance the scope of nutritional and metabolic studies available to the NORC-H investigators. This Core, its Director and Co-Directors, as well as its senior technician will provide: 1. Assistance in the design of successful nutritional stable isotope, and PET experiments including tracer selection, isotope preparation for infusion, and consultation regarding institutional review board approval. 2. A metabolomics platform capable of biomarker and metabolite discovery, biomarker and metabolite identification and metabolite quantitation. 3. Efficient and equitable access to Core analytic instruments, accurate analyses of samples, and quality control. 4. Assistance in the development of new methods for analyses of compounds in biologic samples including blood, urine, tissues and expired air. 5. An opportunity for new investigators to learn about the application of stable isotope-tracer techniques, PET and metabolomics in metabolic/nutritional research.