The Supramolecular Architecture of the Postsynaptic Density

这个项目解决了人类大脑皮层中神经纤维的结构问题。目标1使用的材料来自 手术切除的颞叶进行高分辨率定量电子显微镜研究 兴奋性突触的结构，包括与已确定的中间神经元类别的突触。使用相同的 生物材料，AIM 2进行定量免疫金电子显微镜以定义 突触后密度丰富的不同分子的数量，重点关注其突变是 与神经精神和神经发育疾病有关，尤指精神分裂症。AIM 3表演 工作类似于AIMS 1和2的规定，但重点放在体外培养的皮质有机物质上。这些 这些结果将使比较培养中突触的结构和分子组织成为可能 完整大脑中的突触。

This project addresses the architecture of neuropil in the human cerebral cortex. Aim 1 uses material from surgically-resected temporal lobe to perform high-resolution quantitative electron microscopic studies of the structure of excitatory synapses, including synapses onto identified classes of interneurons. Using the same biological material, Aim 2 performs quantitative immunogold electron microscopy to define the architecture of a number of different molecules abundant in the postsynaptic density, focusing on proteins whose mutations are implicated in neuropsychiatric and neurodevelopmental disease, especially schizophrenia. Aim 3 performs work similar to that specified for Aims 1 and 2, but focusing on cortical organoids cultured in vitro. These results will make it possible to compare the structure and molecular organization of synapses in culture with synapses in intact brain.