Live-Cell Imaging Core

摘要 内布拉斯加州细胞信号中心(NCCS)活细胞成像核心通过咨询服务、试剂和一系列显微技术方面的专业知识，为NCCS内的研究人员和我们的合作机构提供各种支持机制。最初的目标是使先进的显微技术容易地提供给NCCS成员，这一目标继续推动核心的成功，并已扩展到支持更大的研究界。自第二阶段开始形成以来，活细胞成像核心通过为赠款提交和出版物生成数据来支持调查人员。活细胞成像核心由具有共享活细胞成像能力的设施以及针对独特应用的专门功能组成。作为NCCS第三阶段资金的一部分，我们正在申请资金来支持活细胞成像核心的目标。目标1：提供和维护最先进的活细胞显微镜设备，并为NCCS内外的研究人员提供技术支持。目标2：帮助生成图像获取和分析，使研究人员能够将活细胞显微镜纳入他们的想法和他们的项目中，并增加拨款提案获得资助的可能性。目标3：扩大我们中心与其合作机构之间的合作能力。在第二阶段，活细胞成像核心建立了先进显微镜平台所需的技术和系统。与强有力的机构支持一起，我们建议通过在活细胞成像核心内实现TIRF(全内反射荧光)显微镜和Palm(光激活定位显微镜)的超分辨率显微镜能力来继续我们的势头。这些技术的加入将大大提高NCCS调查人员竞争外部资金的能力。活细胞成像核心的长期可持续性将通过以下方式实现：1)保持资金充足的强大用户基础；2)扩大与NCCS以外用户的合作；3)继续提供稳定的机构支持。

Abstract The Nebraska Center for Cellular Signaling (NCCS) Live-Cell Imaging Core provides a variety of support mechanisms to investigators within the NCCS and our partnering institutions through consultation services, reagents, and expertise in an array of microscopy techniques. The original goal of making advanced microscopy technologies readily available to the members of the NCCS continues to drive the success of the core and has been extended to support the larger research community. Since its formation at the beginning of Phase II, the Live-Cell Imaging Core has supported investigators through the generation of data for grant submissions and publications. The Live-Cell Imaging Core is composed of facilities with shared live-cell imaging capabilities as well as specialized features for unique applications. As part of Phase III funding of the NCCS, we are requesting funds to support the aims of the Live-Cell Imaging Core. Aim 1: Provide and maintain state-of-the-art live-cell microscopy equipment and offer technical support to investigators both within and outside the NCCS. Aim 2: Assist in the generation of image acquisition and analysis, allowing investigators to incorporate live-cell microscopy into their thinking and into their projects and increasing the likelihood grant proposals will be funded. Aim 3: Expand the capacity for collaboration between our Center and its partnering institutions. During Phase II, the Live-Cell Imaging Core established the technologies and the systems needed for advanced microscopy platforms. Together with strong institutional support, we propose to continue our momentum by implementing super-resolution microscopy capabilities for TIRF (Total Internal Reflection Fluorescence) microscopy and PALM (Photoactivated Localization Microscopy) within the Live-Cell Imaging Core. Addition of these technologies will greatly enhance the ability of NCCS investigators to compete for extramural funding. Long-term sustainability of the Live-Cell Imaging Core will be achieved by: 1) maintaining a strong base of well-funded users; 2) expansion of collaborations with users outside the NCCS; and 3) continuation of steady institutional support.