IGF::OT::IGF SBIR Topic 355: An Organoid Avatar Biobank for Cancer Disparities. To establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient populatio

这个项目寻求建立一个结直肠癌器官化身生物库，从一个种族和基因多样化的患者群体中，大约40%是非裔美国人。我们将利用被丢弃的、未鉴定的手术标本来建立肿瘤和匹配的正常结肠干细胞(器官)的三维培养。有机化身的特征将是1)WNT、EGF和TGFb(独立)2)结肠癌基因中存在体细胞突变，3)非洲血统，4)测量从批准的肿瘤学药物小组中选择的药物的IC50，以及5)创建具有适合于药物筛选工作的选择结肠癌驱动基因突变的等基因肿瘤有机化身。这项工作将创建有机类线，将用于促进我们对非裔美国人癌症健康差异的基本机制的理解。

This project seeks to establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient population that is approximately 40% African American. We will make use of discarded, de-identified surgical specimens to establish 3-dimensional cultures of tumor and matched normal colon stem cells (organoids). Organoid avatars will be characterized by 1) WNT, EGF, and TGFB (in) dependence 2) presence of somatic mutations in colon cancer genes, 3) percent African ancestry, 4) measure IC50s for select drugs from the Approved Oncology Drugs panel, and 5) create isogenic tumor organoids with select colon cancer driver gene mutations suitable for drug screening efforts. This work will create organoid lines, which will be used to advance our understanding of basic mechanisms of cancer health disparities among African Americans.