Identification of diagnostic and prognostic host biomarkers of Zika virus infection by transcriptome profiling

项目总结 转录组鉴定寨卡病毒感染的诊断和预后宿主生物标志物 剖析 目前，寨卡病毒(ZIKV)在美洲史无前例地持续爆发，还有更多 到目前为止，报告的病例超过10万例。现有诊断测试的局限性包括缺乏特异性 以抗体为基础的检测和非常窄的时间窗口(&lt；1周)。迫切需要更好的诊断测试 需要诊断寨卡病毒感染。此外，综合证据的权重现在表明，ZIKV是 对感染的孕妇的破坏性不良胎儿结局负有直接责任，包括在子宫内 死亡和小头畸形。目前还没有实验室测试来监测受感染的妇女并评估她们的 有发生胎儿并发症的风险。在这里，我们建议使用转录组图谱分析的下一代RNA-Seq 对急性感染患者的血液、尿液和唾液样本进行测序以诊断寨卡病毒感染，以及 从感染寨卡病毒的孕妇连续采集样本以监测宿主的动态 回应。目标是确定可能与活动性相关的诊断和预后宿主生物标志物。 感染(用于诊断)以及孕妇的胎儿感染和结局，可用于 开发监测寨卡病毒感染和疾病的新方法。

PROJECT SUMMARY Identification of diagnostic and prognostic host biomarkers of Zika virus infection by transcriptome profiling There is currently an unprecedented ongoing outbreak of Zika virus (ZIKV) in the Americas, with more than 100,000 cases reported to date. Limitations to existing diagnostic tests include lack of specificity for antibody-based assays and a very narrow time window for PCR (<1 week). Better diagnostic tests are urgently needed to diagnose ZIKV infection. In addition, the weight of the combined evidence now shows that ZIKV is directly responsible for devastating adverse fetal outcomes in infected pregnant women, including in utero demise and microcephaly. There is currently no laboratory test to monitor infected women and assess their risk for fetal complications. Here we propose use transcriptome profiling analysis by RNA-Seq next-generation sequencing of blood, urine, and saliva samples from acutely infected patients to diagnose ZIKV infection, and of serially collected samples from ZIKV-infected pregnant women to monitoring the dynamics of the host response. The goal is to identify diagnostic and prognostic host biomarkers that may be correlated with active infection (for diagnosis) as well as fetal infection and outcomes in pregnant women, and that can be used to develop new assays for monitor ZIKV infection and disease.