Core D: Thymic and peripheral Aspects of T cell Aging and Rejuvenation

核心D-抽象 对传染病的易感性增加是老龄化最突出的影响 免疫系统。除了产生基本的知识，项目1-4的“胸腺和 T细胞老化和再生的外周方面“计划项目赠款(计划)将 提供一系列潜在的年轻化战略。免疫反应& 位于亚利桑那大学的年轻化战略监测核心(核心D)将 为测试这些复兴战略提供最终的实验管道。具体地说， 核心D的全球目标是评估胸腺输出和外周维护 衰老小鼠T细胞返老还童后的幼稚T细胞，并确定这种 干预措施可提高老年人对病毒感染的适应性和保护性免疫 老鼠。 该中心将为专职人员提供适当的传染病专业知识 为确保研究方案执行过程中的一致性和质量控制， 实验程序、动物观察和数据收集，以满足 计划的主要研究目标。这条测试管道不仅会将候选人 干预措施的有效性，但也将确定一些复兴的潜在阶段 这些策略可能无法转化为免疫功能的改善。严谨在体内的使用 分析跟踪最近的胸腺移民，以及对西尼罗河病毒的免疫反应 在老年人中表现出不成比例的高发病率和死亡率的病原体将提供 该计划及其项目对外周免疫功能进行了全面严格的测试 和老年小鼠T细胞返老还童后的保护性免疫。因此，核心将交付 严格的临床前数据，以及人-鼠时间线(由项目生成 1-4和其他核心)将使人类的翻译年轻化努力成为可能。

Core D- Abstract Increased susceptibility to infectious disease is the most prominent repercussion of the aging immune system. In addition to generating basic knowledge, Projects 1-4 of the “Thymic and peripheral aspects of T cell aging and rejuvenation” Program Project Grant (Program) will provide an array of potential rejuvenation strategies. The Immunological Response & Rejuvenation Strategy Monitoring Core (Core D), located at the University of Arizona, will provide the final experimental pipeline for testing those rejuvenation strategies. Specifically, the global objective of Core D is to assess thymic export and peripheral maintenance of naïve T cells following T cell rejuvenation in aged mice, and determine whether such interventions can improve adaptive and protective immunity against viral infection in old mice. The Core will provide dedicated personnel with the appropriate infectious disease expertise to ensure consistency and quality control in the execution of study protocols, application of experimental procedures, and animal observations and data collection necessary to meet the Program's major research objectives. This testing pipeline will not only separate candidate interventions by their efficacy, but will also identify potential stages at which some rejuvenation strategies may fail to translate into improved immune function. The use of rigorous in vivo analysis to track recent thymic emigrants, and immunity in response to West Nile virus, a pathogen that shows disproportionally high morbidity and mortality within the elderly, will provide this Program and its Projects stringent testing of a full spectrum of peripheral immune function and protective immunity following T cell rejuvenation in aged mice. Thereby, the Core will deliver rigorous preclinical data, that, together with the Human-Mouse Timeline (generated by Projects 1-4 and other Cores) will enable translational rejuvenation efforts in humans.