Genomics, Bioinformatics, and Molecular Imaging Core

基因组学，生物信息学和分子成像核心的目标是支持和加强 COBRE和非COBRE研究人员的研究生产力。这是衡量我们的贡献， 他们的同行评议的出版物和补助金申请。我们的客户利用我们的服务，从学习计划， 从设计阶段到数据分析和展示阶段。目前，该中心为以下方面提供服务： 下一代测序（NGS）、DNA桑格测序、基因分型、细胞系验证、生物信息学和 生物统计支持以及其他设备和服务。在第三阶段，我们将进一步加强我们的核心和 在三名共同主任的领导下组织其业务，他们具有互补的专业知识， 提供的服务方面。由于这些增强功能，我们将“分子成像”添加到我们的绰号中。我们 将追求以下具体目标：（1）保持下一代测序（RNA- Seq、WES、miRNA-Seq、Ampli-Seq）、毛细管电泳桑格DNA测序、实时PCR 荧光素酶测定、DNA和RNA分离、定量和质量控制 使用Agilent 2100生物分析仪进行DNA和RNA分析，并为研究者进行细胞系验证。(2)扩大 核心服务的分子组合，并通过建立Visium Spatial Gene来实施新的NGS应用 利用高复合蛋白质组学和全转录组分析，铬单细胞基因 使用10 x Genomics和Illumina系统的表达和Multiome ATAC +基因表达。(3)继续 生物统计学和生物信息学支持核心和核心外产生的数据，并开发新的 生物信息学管道(4)通过实施再充电，扩大和保持核心的可持续性和增长 在第三阶段期间增加，并确定和部署其他支助来源。我们 增强的仪器和扩大的人员将使这成为可能，这将有助于满足日益增长的 增加了对所有这些应用的需求。我们的数据分析服务将允许我们的客户接收数据 这不仅是干净和有效的定量，而且已经通过生物信息学管道， 它在生物学上有着更广泛的意义。核心的生物统计学和生物信息学部分将 有助于开发新的和更好的方法来处理我们的研究人员的数据 生成.

The objective of the Genomics, Bioinformatics, and Molecular Imaging Core is to support and to enhance the research productivity of both COBRE and non-COBRE investigators. This is measured by our contributions to their peer-reviewed publications and grant applications. Our clients utilize our services from the study planning and design phase through the data analysis and presentation phase. The Core currently provides services for next-generation sequencing (NGS), DNA Sanger sequencing, genotyping, cell line validation, bioinformatics and biostatistics support, as well as other equipment and services. In Phase III, we will further enhance our core and organize its operations under three co-directors who have complementary expertise that encompasses all aspects of services offered. Because of these enhancements, we added ‘Molecular Imaging’ to our moniker. We will pursue the following specific aims: (1) Maintain access and services for next-generation sequencing (RNA- Seq, WES, miRNA-Seq, Ampli-Seq), Sanger DNA sequencing with capillary electrophoresis, real-time PCR applications, measurements for luciferase assays, DNA and RNA isolation, quantitation and quality control of DNA and RNA with the Agilent 2100 Bioanalyzer and cell line validation for investigators. (2) Expand the molecular portfolio of core services and implement new NGS applications by establishing Visium Spatial Gene Analysis using High-plex Proteomics and Whole Transcriptome Analysis, Chromium Single Cell Gene Expression and Multiome ATAC + Gene Expression using 10x Genomics and Illumina systems. (3) Continue biostatistics and bioinformatics support for data generated by the core and outside the core and develop new bioinformatics pipelines. (4) Expand and maintain sustainability and growth of the Core, by implementing recharge to all services and increase it over the course of Phase III and identify and deploy other sources of support. Our enhanced instrumentation and expanded personnel will make this possible, and this will help satiate the ever- increasing appetite for all of these applications. Our data analytic services will allow our clients to receive data that is not only clean and valid quantitatively but also has been put through a bioinformatics pipeline that makes it relevant biologically in broader terms. Both the biostatistics and bioinformatics components of the Core will contribute to the development of new and better approaches to the handling of the data our investigators generate.