Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum

澳大利亚黑麦草（Lolium rigum）普遍具有多种除草剂抗性。这种抗性通常是由涉及细胞色素P450的除草剂代谢速率增强（代谢抗性）赋予的。本项目旨在从多个抗除草剂僵菌生物型中鉴定、克隆和表征重要的除草剂代谢P450等基因，并开发用于代谢抗性诊断的转录和生化标志物。除草剂代谢基因的发现、鉴定和标记的开发将极大地扩展目前有限的代谢抗性知识和理解，并有助于实现杂草的可持续管理。

Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and develop transcriptional and biochemical markers for metabolic resistance diagnosis. Herbicide-metabolising gene discovery, characterisation and marker development will greatly extend the currently limited knowledge and understanding of metabolic resistance and help achieve sustainable weed management.