Bioremediation by integrating plant-fungi symbiosis and natural minerals for uptake of metal mixtures

项目摘要 该项目的目标是开发有效的金属生物修复技术 在受采矿遗留影响的场地的受污染沃茨和土壤中出现的混合物， 在纳瓦霍族和拉古纳普韦布洛用于农业和放牧。采矿的负担 活动已经影响了美国的多个超级基金网站，导致多代金属 在我们的合作伙伴社区中曝光。人类接触的有害健康影响 铀、砷、钒和其他共生金属都有很好的记录。化学 影响金属混合物在环境系统中溶解度的反应仍不清楚。 我们的集体专业知识将整合生态学，真菌学， 分子生物学和环境工程。我们将开发新型生物反应器， 通过植物-真菌共生结合吸附和沉淀，使用天然矿物质， 金属混合物的可持续生物修复。 提出了以下具体目标：1。确定相互作用的机制 植物和真菌在水中介导金属的吸附和沉淀 混合物;和2.在土壤中含有植物和真菌的花盆中操作生物反应器， 在与环境相关的水化学处理下，对矿物进行处理，以修复金属混合物。 该研究将为金属混合物的反应性提供新的见解 被真菌和植物感染。与纳瓦霍民族和拉古纳普韦布洛的合作将有助于确保 所产生的科学是对社区需求的回应。此外，该项目旨在 让来自代表性不足的少数群体的研究生参与进来，以这种方式为 培养下一代科学家和工程师。知识来自于 建议的项目是必不可少的风险评估和生物修复风险的进步 金属混合物的还原策略。

Project Summary The goal of this project is to develop effective bioremediation technologies for metal mixtures occurring in contaminated waters and soils in sites impacted by mining legacy that are used for agriculture and grazing in Navajo Nation and Laguna Pueblo. The burden of mining activities has affected various Superfund sites in the U.S., causing multigenerational metal exposures in our partner communities. The negative health impacts of human exposure to Uranium, Arsenic, Vanadium and other co-occurring metals are well documented. The chemical reactions influencing the solubility of metal mixtures in environmental systems remain unclear. Our collective expertise will integrate advances in will integrate advances in ecology, mycology, molecular biology, and environmental engineering. We will develop novel bioreactors catalyzed by plant-fungal symbiosis coupled with adsorption and precipitation using natural minerals for sustainable bioremediation of metal mixtures. The following specific aims are proposed: 1. Identify mechanisms underlying the interaction between plants and fungi in water that mediate the adsorption and precipitation of metal mixtures; and 2. Manipulate bioreactors in pots containing plants and fungi in soils with natural minerals under environmentally relevant water chemistries for remediation of metal mixtures. The proposed research will provide new insights about the reactivity of metal mixtures mediated by fungi and plants. Collaborations with Navajo Nation and Laguna Pueblo will serve to ensure that the science generated is responsive to community needs. Additionally, this project seeks to engage graduate students from under-represented minority groups, in this way contributing to training the next generation of scientists and engineers. The knowledge resulting from the proposed project is essential to risk assessment and to the advancement of bioremediation risk reduction strategies for metal mixtures.