Biospecimen Core

项目摘要/摘要 梅奥诊所乳腺癌孢子的生物谱和病理学核心提供专家 集中的病理咨询服务和乳房组织、血液和“活”的综合资源 用于制备有机物和患者来源的异种移植物的样品，与流行病学和临床相关 数据。Core还对临床上衍生的良性和肿瘤性乳腺组织样本进行了详细的整理 临床病理评估(例如，分期、组织学特征、临床和新兴生物标记物评估) 建立研究登记和队列。采集的样品包括新鲜、新鲜冷冻、超低温保存和 福尔马林固定的石蜡包埋标本来自1)经MAP治疗或未治疗的乳腺癌 背景：良性邻近组织，2)淋巴结转移和远处转移，3)良性乳腺 肿瘤前病变的各种疾病组织和4)从 来自具有或不具有临床或遗传“高危”因素的患者的隆乳/降低风险样本(例如 已知的种系BC易感基因突变)，以及血液。核心执行专家病理 评估与研究相关的组织病理学参数(例如，良性乳腺疾病的异型性、退缩 乳腺小叶的状态、TNBC的组织学亚型、肿瘤对新辅助药物的反应评估 治疗、间质和瘤内TIL评分)。此外，Core还执行抗体优化，如 孢子项目(如PKCBeta1)所要求的，然后对这些项目进行解释和评分 免疫组织化学染色和基于数字图像的方法的应用(例如，优化和 用Aperio(徕卡)(或Halo(Indica Labs)软件系统解释数字图像分析算法， 孢子项目的高复合定量数字空间剖面图(例如，NanoStringGeoMX™平台)。这个 CORE与Mayo Biosecimen Access and Processing(BAP)共享资源协调以存储 新鲜冷冻和冷冻保存的标本，并处理血液样本以提供基因组DNA和血清 和血浆等量；它与梅奥病理研究核心(PRC)共享资源合作，以获得 以组织为基础的基本技术服务，包括常规组织切片和低温组织切片， 免疫组织化学、组织微阵列构建、载玻片扫描和细胞技术专家协助 优化数字成像算法。与这些机构共享资源合作可最大限度地减少 服务冗余，并利用技术经验和最先进的技术。

PROJECT SUMMARY/ABSTRACT The Biospecimen and Pathology Core of the Mayo Clinic Breast Cancer SPORE provides an expert centralized pathology consultative service and a comprehensive resource of breast tissues, blood and “living samples” for preparation of organoids and patient-derived xenografts, linked to epidemiological and clinical data. The Core also curates clinically derived benign and neoplastic breast tissue samples with detailed clinicopathological evaluation (e.g., stage, histologic features, clinical and emerging biomarker assessments) to establish registries and cohorts for research. Samples collected include fresh, fresh-frozen, cryopreserved and formalin-fixed paraffin embedded specimens from 1) treated or untreated breast cancers with mapped background “benign-adjacent” tissues, 2) lymph node metastases and distant metastases, 3) benign breast disease tissues across the spectrum of pre-neoplasia and 4)“normal” breast tissue harvested from mammoplasty/risk reduction specimens from patients with or without clinical or genetic “high-risk” factors (e.g. known germline BC predisposition gene mutations), as well as blood. The Core performs expert pathological evaluation of research- related histopathological parameters (e.g., atypia in benign breast disease, involution status of breast lobules, histologic subclassification of TNBC, evaluation of tumor response to neoadjuvant therapy, stromal and intratumoral TIL scoring). In addition, the Core performs antibody optimizations as required by the SPORE projects (e.g. PKCBeta1), followed by interpretation and scoring of these immunohistochemical stains, and application of digital image-based approaches (e.g., optimization and interpretation of digital image analysis algorithms with Aperio (Leica) (or HALO (Indica Labs) software systems, high-plex quantitative digital spatial profiling (e.g. Nanostring GeoMX™ platform) for SPORE projects. The Core coordinates with the Mayo Biospecimen Accessioning and Processing (BAP) Shared Resource to store fresh-frozen and cryopreserved specimens and to process blood samples to provide genomic DNA and serum and plasma aliquots; it collaborates with the Mayo Pathology Research Core (PRC) Shared Resource to obtain essential technical tissue-based services including conventional and cryostat tissue sectioning, immunohistochemistry, tissue microarray construction, slide scanning and cytotechnologist assistance for optimizing digital imaging algorithms. Partnering with these institutional shared resources minimizes redundancy of services and capitalizes both on technical experience and state-of-the-art technologies.