Research Capacity Core

项目摘要。Meharry RCMI研究能力核心(MRRCC)的总体目标是提高 梅哈里医学院资助的研究人员数量。为此，RCMI配备了设备和工作人员 MRRCC拥有最先进的生物医学仪器，使我们的研究人员能够在 与范德比尔特大学等研究密集型机构的调查人员地位平等。MRRCC核心 董事们还与范德比尔特大学的同行建立了密切的工作关系 我们的调查人员能够在范德比尔特获得他们可能需要但目前无法使用的设备 在梅哈里。例如，我们的调查人员可以访问并利用范德比尔特的电子显微镜进行他们的研究 由于这种安排而产生的成像需求(见支持函)。在RCMI当前的资金周期内，我们的 研究人员能够获得R01、R21、SC1和U54-癌症研究人员发起的资金，基于 在MRRCC中生成的数据。在MRRCC的帮助下获得的这些非RCMI赠款总数更多 而不是11,000,000美元。MRRCC还促进了我们调查人员之间的合作。例如，在 在当前的RCMI资助周期中，314项基础科学资助申请至少与5个MRRCC中的一个合作 核心。具体地说，65个应用程序与一个核协作，50个应用程序与2核协作，54个应用程序与3核协作，37个应用程序与4核协作 8个核心与所有5个核心协同工作。在目前的应用中，我们对MRRCC有两个广泛的具体目标： 目标1.拓展核心服务和资源。我们将用机构资金购买OpenSPR系统，用于 蛋白质-蛋白质实时相互作用和代谢组学研究的海马体。随着资金的到位，我们将 将继续根据我们用户的需求提供新的仪器。我们将建立一个新的基因组数据 核心。该核心将提供下一代测序(NGS)数据分析、数据挖掘、AI/ML和 通过开发基因组数据库和最先进的数据访问门户网站实现可视化。最后，我们将 建立基因编辑服务。新的核心服务将帮助用户设计CRISPR/CAS9 实验方案，进行转染/感染，确定CRISPR效率，克隆阳性细胞，以及 评估目标上/目标外的效率。 目标2.继续提供优质服务。我们提供的服务被认为是产生 赠款的初步数据，在影响较大的期刊上发表手稿，以及使校外采购成为可能 给予支持。我们将加强业务运营。我们正在与我们的办公室建立直接连接 信息技术(OIT)提供无缝数据跟踪，并通过在 制度层面的制度。我们还将加强MRRCC分核心内的互联互通，Meharry 研究部门和国家RCMI网络。我们会加强收回成本的工作，要求 资助调查人员每年至少预算2,000美元，用于他们将从MRRCC获得的服务。

Project Summary. The overarching goal of Meharry RCMI Research Capacity Core (MRRCC) is to increase the number of funded investigators at Meharry Medical College. To this end, RCMI has equipped and staffed MRRCC with the latest state of the art biomedical instrumentation that enables our investigators to compete on equal footing with investigators from research intensive institutions like Vanderbilt University. MRRCC Core directors have also established a close working relationship with their counterparts at Vanderbilt University such that our investigators are able to access equipment at Vanderbilt that they may need but not currently available at Meharry. For example, our investigators can access and utilize electron microscope at Vanderbilt for their imaging needs due to such arrangements (see letter of support). During the current funding cycle of RCMI, our investigators were able to obtain R01, R21, SC1 and U54-Cancer investigator-initiated funding based on the data generated in MRRCC. These non-RCMI grants that were obtained with the help of MRRCC totaled more than $ 11,000,000. MRRCC has also fostered collaboration among our investigators. For example, during the current RCMI funding cycle, 314 basic science grant applications collaborated with at least one of the 5 MRRCC cores. Specifically, 65 applications collaborated with one core, 50 with 2 cores, 54 with 3 cores, 37 with 4 cores and 8 collaborated with all the 5 cores. We have 2 broad specific aims for MRRCC in the current application: Aim 1. Expand core services and resources. We will purchase with institutional funds an OpenSPR system for protein-protein interactions in real time and Seahorse for metabolomics studies. As funds become available, we will continue to provide new instrumentation based on our user’s needs. We will establish a new Genomics Data Core. This core will provide Next Generation Sequencing (NGS) data analysis, data mining, AI/ML, and visualization through development of genomic databases and state-of-the-art data-access portal. Lastly we will establish Gene Editing Services. The new core service will help users with the design of CRISPR/Cas9 experimental protocols, perform transfection/infection, determine CRISPR efficiency, clone positive cells, and evaluate on/off-targets efficiency. Aim 2. Continue to provide high quality service. The services we provide are deemed necessary for generating preliminary data for grants, publishing manuscripts in high impact journals, and enabling acquisition of extramural grant support. We will enhance business operations. We are establishing direct connectivity with our Office of Information Technology (OIT) to provide seamless data tracking and improve cost recovery by connecting within institutional-level systems. We will also enhance interconnectivity within the MRRCC Sub-Cores, Meharry research departments, and the national RCMI network. We will enhance cost recovery efforts by requiring funded investigators to budget at least $ 2,000 per year for services that they will get from MRRCC.