Transgenic and Gene Targeting Core

转基因小鼠已成为生物医学研究的重要工具。它们是关键的工具， 研究皮肤及其附属物的发育。此外，这些老鼠模型已经成为 对于建立人类皮肤病模型是必不可少的，包括遗传性和获得性皮肤病和 皮肤癌。生产转基因或基因敲除小鼠需要专门的技能和昂贵的设备。 因此，很少有实验室有能力产生这些有价值的研究模型。转基因生物 科罗拉多大学丹佛分校的基因打靶(TGT)核心提供一套全面的服务 这使研究人员能够培育出基因工程小鼠。这些服务包括生成 通过原核注射转基因小鼠，以及基因打靶实验，包括从 产生重组ES细胞以产生嵌合体小鼠。调查人员所需的一切都是为了 启动这些实验的载体是转基因载体或基因打靶载体。此外，我们的核心提供广泛的 帮助调查人员制定研究计划的咨询服务，包括病媒设计， 动物设计，以及对转基因小鼠的分析。23名皮肤病研究人员 核心中心(UCD-SDRC)正计划使用我们的核心服务来生成用于研究皮肤生物学的老鼠模型 或者皮肤病。核心中心对我们核心的支持将为这些调查人员提供补贴访问 我们的全套服务，从而方便他们的研究。

Genetically engineered mice have become an essential tool in biomedical research. They are crucial tools for studying development of the skin and its appendages. In addition, these mouse models have become indispensible for creating models of human skin disorders, including inherited and aquired skin disorders and skin cancer. Generating transgenic or knockout mice requires specialized skills and expensive equipment. Consequently, very few laboratories have the ability to generate these valuable research models. The Transgenic and Gene Targeting (TGT) Core at the University of Colorado Denver offers a comprehensive set of services which enables investigators to generate genetically engineered mice. These services include the generation of transgenic mice via pronuclear injections, and gene targeting experiments that include the entire process from generating recombinant ES cells to the production of chimeric mice. All that is required from the investigators to initiate these experiments is a transgenic vector or gene targeting vector. Furthermore, our core offers extensive consultation services to help investigators with the development of a research plan, including vector design, animal design, and the analysis of genetically engineered mice. 23 investigators of the Skin Disease Research Core Center (UCD-SDRC) are planning to use our core services to generate mouse models to study skin biology or skin diseases. Core Center support for our core will provide these investigators with subsidized access to the full set of our services, and thus facilitate their research.