Stanford Tissue Mapping Center

摘要：器官专案 除了吸收营养外，胃肠道对正常人的影响也很广泛。 和其他器官系统的疾病生理，包括新陈代谢、神经功能和 免疫系统。一张高分辨率的肠道地图将是一项宝贵的资源 了解正常的肠功能和导致疾病的紊乱。我们建议 沿着小肠和结肠的长度创建这张地图，这两者都有细微的差异 功能的地理专门化。使用单细胞RNA-seq和单细胞atac-seq，我们 将描述定义复杂细胞的基因表达和调控程序 推动肠道功能的人群。我们将使用Codex，一种高度多元化的、基于抗体的 作图方法，以确定这些细胞群体的空间关系。这些 将对以某种方式保存和获取的组织进行调查 适用于人体肠道移植。是目标里程碑是描述小肠的特征 在这四年的努力过程中，总共从22个人身上提取了结肠组织。我们的 从其家人提供广泛、开放的人体器官捐赠者那里获取组织的议定书 访问许可已经到位，我们正在积极收集组织用于其他研究。

ABSTRACT: Organ Specific Project Beyond nutrient absorption, the gastrointestinal tract has wide-ranging effects on the normal and diseased physiologies of other organ systems, including metabolism, neural function, and the immune system. A high-resolution map of the bowel would be an invaluable resource to understand normal bowel function and the perturbations that lead to disease. We propose to create this map along the length of the small bowel and colon, both of which have nuanced geographic specializations of function. Using single cell RNA-seq and single cell ATAC-seq, we will profile the gene expression and regulatory programs that define the complex cell populations that drive bowel function. We will use CODEX, a highly-multiplexed, antibody-based mapping method, to define the spatial relationships of these cell populations. These investigations will be performed on tissues that are preserved and procured in a manner suitable for human bowel transplantation. Are target milestone is to characterize the small bowel and colon tissues from a total of 22 individuals over the course of this four-year effort. Our protocol to obtain tissues from human organ donors whose families have provided broad, open access consent is in place, and we are actively collecting tissues for other studies.