Morphology and Imaging Module

总结/摘要-形态学和成像模式 形态学和成像模块的主要功能是为专家人员提供访问权限， 用于固定和活眼组织显微镜研究的设备。共有921间客房 平方英尺专用空间，该模块通过提供最先进的技术来增强研究环境 为那些使用解剖和成像技术的研究人员提供设备，而且，由于技术 它提供了专业知识，使其他人能够将解剖技术加入他们的研究工具库。 提供了用于以下操作的仪器的访问和培训：1）处理眼组织以获得光， 共聚焦显微镜、免疫组织化学和原位杂交，以及2）捕获和处理 通过光和共焦（常规和多光子）显微镜观察活体和固定组织的图像。 该模块中可用的设备包括两个低温恒温器，一个石蜡处理和包埋站， 石蜡切片机，Leica激光显微切割显微镜，两个宽视场荧光显微镜（一个 Olympus EX-51显微镜和Leica DM 6000显微镜）、常规的Leica TCS SP 5共聚焦显微镜（例如， 显微镜和多光子Leica TCS SP 5共聚焦显微镜。此外，还有一台电脑 专用于图像分析的工作站，配备Leica AF、MetaMorph、Imaris和其他 成像软件。 该模块配备了一名全职技术人员，负责协助处理和切片 组织，实验设计，光和荧光显微镜的使用培训， 相关成像软件和仪器维护。还为模块配备人员（20%的工作量，由 眼科学系）是密歇根大学形态学和图像分析中心的经理。 糖尿病和研究培训中心，提供徕卡SP 5共聚焦显微镜使用培训 显微镜和Leica AF、MetaMorph和Imaris成像软件。形态学和成像模块 在过去5年中，17名参与研究者中度或广泛使用， 预期在下一个资助期内适度或广泛使用该单元。

SUMMARY/ABSTRACT - MORPHOLOGY AND IMAGING MODULE The primary function of the Morphology and Imaging Module is to provide access to expert personnel and equipment for microscopic studies of both fixed and live ocular tissues. Housed in a suite of rooms totaling 921 sq. ft. of dedicated space, this Module enhances the research environment by making available state-of-the-art equipment for those investigators who use anatomical and imaging techniques, and, because of the technical expertise provided, it enables others to add anatomical techniques into their repertoire of research tools. Access and training are provided for instrumentation used in 1) the processing of ocular tissue for light and confocal microscopy, immunohistochemistry, and in situ hybridization, and 2) the capturing and processing of images of living and fixed tissue by light and confocal (conventional as well as multiphoton) microscopy. Equipment available in this module includes two cryostats, a paraffin processing and embedding station and paraffin microtome, a Leica laser microdissection microscope, two wide-field fluorescence microscopes (an Olympus EX-51 microscope and a Leica DM6000 microscope), a conventional Leica TCS SP5 confocal microscope, and a multiphoton Leica TCS SP5 confocal microscope. In addition, there is a computer workstation dedicated to image analysis with a stand-alone version of Leica AF, MetaMorph, Imaris and other imaging software. The Module is staffed by a full-time technician who provides assistance with the processing and sectioning of tissue, the design of experiments, training in the use of light and epifluorescence microscopes and associated imaging software, and maintenance of instruments. Also staffing the module (20% effort, paid by the Department of Ophthalmology) is the manager of the Morphology and Image Analysis Core of the Michigan Diabetes and Research Training Center, who provides training in the use of the Leica SP5 confocal microscopes and Leica AF, MetaMorph, and Imaris imaging software. The Morphology and Imaging Module was moderately or extensively utilized by 17 participating investigators during the past 5 years and 16 anticipate using the Module to a moderate or extensive degree in the next funding period.