DNA Microarray Data Analysis

本研究探讨了五溴联苯醚（PBDE）暴露对出生后第22天Wistar Han大鼠幼鼠的影响。PBDE-47是一种PBDE混合物（DE-71）的同系物，该混合物被选择作为测试PBDE同系物，以比较PBDE-47和DE-71的毒性。在子宫和出生后，通过灌胃使用浓度分别为0、0.1、15和50 mg/kg的化学物质暴露。PBDE-47和DE-71暴露均可导致PND 22引起的小叶中心肥大和肝脏脂肪改变，并降低血清甲状腺素水平。肝脏转录组学变化包括诱导细胞色素p450转录本水平、Nrf2抗氧化途径转录本上调和ABC膜运输转录本上调。这些转录变化表明，长期暴露于PBDE-47和DE-71可能是肝脏毒素或致癌物。基因组基准剂量浓度计算表明，PBDE-47暴露导致的基准剂量浓度低于DE-71暴露。

We explored the effect of pentabromodiphenyl ether (PBDE) exposure on Wistar Han rats pups on postnatal day 22. PBDE-47 is a congener of a PBDE mixture (DE-71) that was chosen as the test PBDE congener to compare PBDE-47 toxicity to the toxicity of DE-71. In utero and postnatal exposure was performed using chemical concentrations of 0, 0.1, 15, and 50 mg/kg by gavage. Both PBDE-47 and DE-71 exposure led to centrilobular hypertrophy and fatty change in liver and reduced serum thyroxin levels by PND 22. Liver transcriptomic changes included induced cytochrome p450 transcript levels and up-regulation of Nrf2 antioxidant pathway transcripts and up-regulation of ABC membrane transport transcripts. These transcriptional changes suggest that exposure to PBDE-47 and DE-71 could be a liver toxin or carcinogen after long-term exposure. A genomic benchmark dose concentration calculation showed that PBDE-47 exposure led to lower benchmark dose concentration than exposure to DE-71.