System for Multiplex Protein:Protein Interaction Studies Modeled with Antibodies

描述(由申请人提供)：第一阶段的主要目标是产生一种方法，该方法将在一次快速实验中生成、鉴定和克隆蛋白质-蛋白质相互作用的目录。在这项提案中，我们将使用抗体-抗原相互作用来模拟这项技术。针对蛋白质组的重组抗体库可以在任何需要之前建立，然后根据需要系统地克隆所需的亲和试剂。针对蛋白质组的重组生物扫描整合产物的文库也可以与针对一组条形码的引物组一起分布，以便于克隆。在未来的努力中，该方法将被用于克隆肿瘤细胞或其他疾病组织的cDNA，以用作表位库，以快速识别潜在的生物标记物。这一阶段的成功完成将使抗体商业化，这些抗体可用于诊断和治疗应用，其速度和全面性是目前方法不易实现的。由载体、试剂、菌株和方法组成的试剂盒将具有商业价值。

DESCRIPTION (provided by applicant): The overarching goal of Phase I is to produce a method that will generate, identify and clone a catalog of protein-protein interactions in a single rapid experiment. In this proposal we will model the technology using antibody-antigen interactions. Recombinant antibody libraries against a proteome could be produced prior to any need, and then desired affinity reagents systematically cloned as needed. Libraries of recombinational biopanning integration product against a proteome could also be distributed along with primer sets against a set of barcodes for facile cloning. In future endeavors, the method will be used to clone tumor-cell or other diseased tissue cDNAs to use as an epitope library to quickly identify potential biomarkers. Successful completion of this phase will allow th commercialization of antibodies that can be used for diagnostic and therapeutic applications at a speed and comprehensiveness that is not easily achieved by current methods. Kits composed of vectors, reagents, strains and methods would be of commercial interest.