Novel microfluidic platform for versatile, fast, low-cost DNA synthesis and assembly

项目总结 ELegen Corporation提议开发一种新型仪器，这种仪器将快速且廉价地合成 DNAElegen的方法利用了一个创新的微流控平台，使我们能够创建一个简单的桌面 可以在实验室中部署的系统。许多生物医学应用的开发-以及 整个合成生物学领域-通常涉及迭代通过设计-构建-测试循环，需要 每一次迭代都有大量定制的合成DNA。订货和订货之间的周转时间(TAT) 从供应商那里收到DNA产品可能需要几周时间，因此仍然是这方面的关键瓶颈 循环播放。这种长时间的纹身严重阻碍了新诊断和治疗的发展步伐。Elegen‘s 技术将通过提高完成订单的灵活性将TAT从几周缩短到几天，并将减少 成本。这些改进将对研究效率产生革命性的影响，并将使科学家能够 将生物医学创新带给患者，速度比目前可能的要快得多。伊莱根已经分开了 演示了实施拟议系统所需的所有关键技术组件。在这个项目中， 该公司将集成和优化这些组件，并展示全功能原型 系统。

PROJECT SUMMARY Elegen Corporation proposes to develop a novel instrument that will quickly and inexpensively synthesize DNA. Elegen's approach exploits an innovative microfluidic platform, enabling us to create a simple benchtop system that can be deployed in the laboratory. Development of many biomedical applications – as well as the entire field of synthetic biology – typically involves iterating through a design-build-test loop requiring large quantities of custom synthetic DNA with every iteration. The turnaround time (TAT) between ordering and receiving DNA products from vendors can be several weeks and thus remains as the critical bottleneck in this loop. This long TAT severely impedes the pace of development of new diagnostics and therapeutics. Elegen's technology will shorten TAT from weeks to days by increasing flexibility in fulfilling orders and will also reduce cost. These improvements will have a transformative impact on research efficiency and will enable scientists to bring biomedical innovations to patients much faster than is currently possible. Elegen has already separately demonstrated all the key technology components needed to implement the proposed system. In this project, the company will integrate and optimize these components and demonstrate a fully-functional prototype system.