Core 2: Data Analysis and Translational Application Core

数据分析和翻译及翻译应用核心（DATA Core） AACORT的成立是为了1）提供一个标准化计算和 人类高通量测序数据的生物信息学分析，以及2）提供基础设施 用于高维患者匹配数据的标准化注释、存储和共享。作为 AACORT对新发斑秃患者进行转化研究， 项目成员将需要一种标准化的方式来访问和分析数据，以确保 资源的有效利用。DATA Core将提供这些服务，定制数据分析， 研究项目的需要。它还将作为与核心预算资源调拨目标核心资源的接口，核心资源调拨目标将 主要负责患者采集和样本处理的临床方面。 通过TRAC核心的临床部门获得的生物样本将被处理， 相应的测序服务，并且数据和所有临床注释将被传递到 用于处理、存储和后续分析的数据核心。此外，数据核心将 在随后的试点实验和研究设计中与TRAC核心合作 项目，以确保适当的实验设计，提供适当的权力，为计算 分析。在具体的服务方面，数据核心及其人员配备了两个 培训和设施，以处理广泛的数据，包括：基因分型/外显子组 测序、转录组学和调控建模以及免疫蛋白质组学/蛋白质结构 分析.这将包括体细胞变体鉴定、靶向外显子组测序、生物标志物 开发，掌握监管分析和基于网络的药物预测。它还将包括 用于抗原和TCR测序的免疫分型、计算抗原预测和利用 病原性免疫细胞的单细胞测序。我们召集了一个训练有素， 位于哥伦比亚大学医学院的多学科科学家和调查人员团队 系统生物学，生物物理学和计算机科学，以补充生物，临床和 AACOT其他模块的免疫学专业知识。我们还得到以下方面的支持： 哥伦比亚核心设施，以及中心的广泛的计算基础设施， 计算生物学和生物信息学和系统生物学系，以促进 存储和分析我们的数据。

The Data Analysis and Translational and Translational Applications Core (DATA Core) of the AACORT was established to 1) provide a core center for standardized computational and bioinformatic analysis of human high-throughput sequencing data, and 2) provide an infrastructure for the standardized annotation, storage, and sharing of high-dimensional, patient matched data. As the AACORT pursues translational research on new-onset Alopecia Areata patients, the Resource Project Members will need a standardized manner of accessing and analyzing the data to ensure efficient use of the resources. The DATA Core will provide these services, tailoring data analysis to the needs of the Research Project. It will also serve as an interface with the TRAC Core, which will primarily be responsible for the clinical aspects of patient acquisition and sample processing. Biosamples acquired through the clinical arms of the TRAC Core will be processed for corresponding sequencing services, and the data and all clinical annotations will be passed to the DATA Core for processing, storage, and subsequent analysis. Additionally, the DATA Core will collaborate with the TRAC Core in the subsequent design of pilot experiments and research projects to ensure proper experimental design that provides proper power for the computational analyses. In terms of specific services, the DATA Core and its personnel are equipped both by training and in facilities, to handle a broad spectrum of data including: genotyping/exome sequencing, transcriptomics and regulatory modeling, and immunoproteomics/protein structure analysis. This will include somatic variant identification, targeted exome sequencing, biomarker development, master regulatory analysis and network-based drug predictions. It will further include immunotyping for antigen and TCR sequencing, computational antigen predictions, and leveraging of single-cell sequencing for pathogenic immune cells. We have assembled a highly trained, multidisciplinary team of scientists and Investigators at the Columbia University Medical Campus in Systems Biology, Biophysics, and Computer Science to complement the biological, clinical and immunological expertise of the other modules of the AACORT. We further have the support of Columbia Core Facilities, as well as the extensive computational infrastructure of the Center for Computational Biology and Bioinformatics and the Department of Systems Biology to facilitate the storage and analysis of our data.