Cell Engineering Shared Resource

细胞和病毒载体实验室共享资源 项目摘要 细胞和病毒载体实验室(CVVL)共享资源的使命是服务于 WAKE森林浸信会综合癌症中心(WFBCCC)的研究人员通过提供定制的 协助专门的细胞培养和病毒载体的生产；并通过为每个 研究项目。使用来自癌症和非癌症来源的原代和已建立的培养物是 对癌症研究至关重要，需要仔细的质量控制，以保持适当的表型和 功能。此外，各种病毒载体(包括慢病毒、逆转录病毒和腺病毒)的使用已经 成为癌症研究的关键实验技术，以有效地操纵基因表达和 在特定基因中产生所需的突变。CVVL也是购买和 以较低的成本分发特定的培养介质/试剂、分子生物学试剂盒和标准实验室用品- 为WFBCCC成员提供有效的礼仪。CVVL的具体目标是：1)提供专门的细胞培养 以高效和经济高效的方式支持WFBCCC成员的研究需求的服务；2) 维护常用细胞系细胞库，供WFBCCC成员使用；3)提供WFBCCC 成员获得个人研究人员不容易获得或成本效益不高的专门设备； 4)作为关键细胞培养和分子生物学试剂的集散地。CVVL是 被139多名首席调查员广泛使用(包括WFBCCC成员和非成员) 每年；63%的服务支持WFBCCC成员的研究。为调查人员提供服务 在所有院校校园内，在严格的标准下开展工作，节省大量成本 操作程序。CVVL主任Purnima Dubey博士的专业知识和两个人的努力 技术人员(1.5 FTE)为研究人员提供高质量的细胞培养和病毒载体生产服务 WFBCCC。未来，CVVL计划通过扩展其尖端技术来扩展病毒生产服务 病毒载体系统。

CELL AND VIRAL VECTOR LABORATORY SHARED RESOURCE Project Summary The mission of the Cell and Viral Vector Laboratory (CVVL) Shared Resource is to serve the scientific needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers by offering customized assistance for specialized cell culture and viral vector production; and by providing quality control for each research project. The use of primary and established cultures from cancerous and non-cancerous origins is essential for cancer research and requires careful quality control to maintain appropriate phenotype and functions. In addition, the use of various viral vectors (including lentivirus, retrovirus and adenovirus) has become a critical experimental technique for cancer research to effectively manipulate gene expression and to create desired mutations in particular genes. The CVVL also serves as a central site for purchasing and distributing specific culture media/reagents, molecular biology kits, and standard laboratory supplies in a cost- effective manner for WFBCCC members. The Specific Aims of CVVL are to 1) provide specialized cell culture services that support WFBCCC members' research needs, in an efficient and cost-effective manner; 2) maintain a cell repository of commonly used cell lines for WFBCCC member use; 3) provide WFBCCC members access to specialized equipment not readily available or not cost-effective for individual researchers; and 4) serve as a distribution center for critical cell culture and molecular biology reagents. The CVVL is extensively utilized by more than 139 Principal Investigators (both WFBCCC members and non-members) annually; 63% of services support the research of WFBCCC members. Services are provided to investigators on all institutional campuses at substantial cost savings with work conducted under stringent standard operating procedures. The expertise of the CVVL Director, Purnima Dubey, Ph.D., and the efforts of two technicians (1.5 FTE) provide high-quality cell culture and viral vector production services to researchers at WFBCCC. In the future, the CVVL plans to extend the viral production service by expanding its cutting-edge viral vector systems.