A multiphoton confocal microscope for biomedical research at Purdue University

描述（由申请人提供）：普渡大学已经确定了一些美国国立卫生研究院资助的研究人员对最先进的多光子显微镜的迫切需求。目前在普渡大学，没有多光子激发显微镜可供普通用户使用，包括被列为主要用户的美国国立卫生研究院资助的研究人员。缺乏可用的多光子显微镜一直是一个主要的限制因素，在原位成像研究的研究者。成像需求的一些关键例子是：1)肠道和相关淋巴组织中粘膜FoxP3+调节性T细胞的运输；2)血液循环中罕见肿瘤细胞检测；3)血小板与体内免疫/组织细胞的相互作用；4)骨髓肿瘤抑制细胞与肿瘤细胞的体内相互作用；5)腺病毒介导的体内基因转移动力学；6)宿主细胞与布鲁氏菌体内相互作用；7)三维培养乳腺上皮细胞向腺泡结构发育的动态过程；8)免疫细胞在体内对注射疫苗的募集和相互作用。所有这些研究项目都旨在改善人类健康。我们恭敬地请求拨款购买最先进的多光子显微镜。该系统能够将发射荧光聚焦在焦点体积内，并使用超快红外光进行多光子激发，因此该系统具有许多理想的功能，可用于在生理环境中对3D培养物和动物中的活细胞进行原位成像。普渡大学完全致力于通过提供空间、资源以及技术和培训支持方面的FTE来支持该设备的采购。技术人员和调查人员具有高水平的技术专长，可以有效地使用所要求的多光子显微镜系统。多光子显微镜系统将通过宾德利生物科学中心和普渡大学多光子咨询委员会的专业管理来保持最佳状态，以便由美国国立卫生研究院资助的研究人员和普渡大学的其他人员有效和安全地使用。如果获得资助，多光子显微镜将极大地改善普渡大学的生物医学研究环境，并为人类健康的进步做出贡献。

DESCRIPTION (provided by applicant): Purdue University has identified an urgent need for a state-of-the-art multiphoton microscope by a number of NIH-funded investigators. Currently at Purdue University, there is no multiphoton excitation microscope available for general users including the NIH-funded investigators listed as major users. The lack of an available multiphoton microscope has been a major limiting factor for in situ imaging research of the investigators. Some key examples of the imaging needs are 1) trafficking of mucosal FoxP3+ regulatory T cells in the gut and associated lymphoid tissues; 2) detection of circulating rare tumor cells in blood; 3) interaction of platelets and immune/tissue cells in vivo; 4) interaction of myeloid tumor suppressor cells with tumor cells in vivo; 5) kinetics of adenoviral mediated gene transfer in vivo; 6) host cell and Brucella interaction in vivo; 7) the dynamic process of mammary epithelial cell development into the acinar structure in 3D culture; and 8) immune cell recruitment to and interaction with injected vaccines in vivo. All of these research projects are aimed to improve human health. We respectfully request for funding to acquire a state- of-the art multiphoton microscope. With the capability to keep emission fluorescence focused within the focal volume and use of an ultrafast infrared light for multiphoton excitation, the system has a number of desirable features for in situ imaging of live cells in 3D cultures and animals in a physiological setting. Purdue University is fully committed to support the acquisition of this equipment by providing space, resources, and FTE in tech and training support. The technical staff and investigators have the high-level technical expertise appropriate for effective use of the requested multiphoton microscope system. The multiphoton microscope system will be maintained in top shape by professional management through the Bindley Bioscience Center and the Purdue multiphoton advisory committee for effective and safe use by the NIH-funded investigators and others at Purdue. If funded, the multiphoton microscope will greatly improve the biomedical research environment at Purdue University and contribute to the advancement of human health.