OLYMPUS FV1000 INVERTED SPECTRAL CONFOCAL SYSTEM

描述(申请人提供)：共聚焦显微镜已成为许多生物医学研究项目的重要组成部分。麦克劳克林研究所上一次收购共焦单位(Bio-Rad MicroRadiance)是在12年前。它有多个限制，包括两种颜色，感光度差，以及缺乏制造商支持。在其他研究机构进行共聚焦显微镜检查需要三个多小时的单程车程。这项申请申请资金奥林巴斯FV1000光谱共聚焦与IX81倒置显微镜和附件。这种显微镜将允许对周围神经切片、被梳理的神经纤维、神经球、脑切片和通过原位杂交、免疫荧光、生命染料和标记蛋白质标记的胚胎进行成像。这些方法对于推进NIH资助的项目至关重要，这些项目研究神经系统损伤和再生、阿尔茨海默病、帕金森病、脱髓鞘疾病和周围神经病的潜在机制。另一台显微镜的加入也将缓解日程冲突，并允许进一步扩大规模，计划在未来两年增加两名教员。

DESCRIPTION (provided by applicant): Confocal microscopy has become an important part of many biomedical research programs. The McLaughlin Research Institute last acquired a confocal unit (Bio-Rad MicroRadiance) twelve years ago. It has multiple limitations, including two colors, poor sensitivity, and lack of manufacturer support. Performing confocal microscopy at other research institutions requires more than a three-hour drive one way. This application requests funds for an Olympus FV1000 Spectral Confocal with IX81 inverted microscope and accessories. This microscope will allow imaging of peripheral nerve sections, teased nerve fibers, neurospheres, brain sections, and embryos labeled by in situ hybridization, immunofluorescence, vital dyes, and tagged proteins. These methods are critical to advancing NIH-funded projects studying mechanisms underlying nervous system injury and regeneration, Alzheimer's disease, Parkinson's disease, demyelinating diseases, and peripheral neuropathies. The addition of another microscope also will alleviate scheduling conflicts and allow for further expansion, with two new faculty planned in the next two years.