IVIS Spectrum Imaging System

描述（由申请人提供）：在研究环境中，先进的光学成像设备有潜力扩大非侵入性成像在科学发现中的作用。我们建议购买最先进的生物发光/荧光成像系统（IVIS光谱）。在比较了现有的系统后，我们决定卡钳生命科学公司（马萨诸塞州波士顿）的IVIS光谱最能满足我们研究界的需求。这样的系统不仅将大大提高我们的体内光学成像能力，而且还允许与磁共振成像（MRI）和高分辨率微CT扫描收集的数据集成。加州大学圣地亚哥分校拥有世界上最先进的功能性核磁共振成像设备之一，这些互补的成像方式将使研究人员能够在以前无法实现的水平上进行研究。定位于三维定量解剖结构的信号将提高从正常发育到疾病病理研究的精度。此外，有许多类型的实验可以设计成离体组织和细胞成像，并利用该成像系统进行高通量筛选。重要的是，该光学成像设备将成为正在开发的多模块生理学核心的一部分，用于提供小动物功能分析，包括心血管动力学，代谢分析和肺功能。这些多个小动物功能模块的支持将补充成像模式，以更好地了解收集到的成像数据的生理基础。医学院医学教学设施社区（尽管其名称主要是一个以研究为导向的社区）迫切需要一个综合资源，将非侵入性成像数据与评估综合生理功能变化的方法结合起来。所有MTF研究人员都可以使用这种最先进的光学成像数据采集方法、软件，以及数据处理、图像分析和数据集成方法的培训和教育。随着采集和分析技术的加速发展，研究人员将光学成像纳入其研究项目的核心将为组成加州大学圣地亚哥分校社区的研究人员提供一个知识库，将使这些新工具和成像技术广泛可用，并鼓励更广泛地使用这些技术。加州大学圣地亚哥分校和更大的圣地亚哥社区拥有强大的研究项目，这个成像中心将增加研究人员实现甚至超越他们的研究目标的能力。

DESCRIPTION (provided by applicant): In research settings advanced optical imaging facilities have the potential to expand the role of non- invasive imaging for scientific discovery. We are proposing to purchase a state of the art bioluminescent/fluorescent imaging system (the IVIS Spectrum). After comparing available systems we have decided that the IVIS Spectrum from Caliper Life Sciences (Boston MA), best serves the needs of our research community. Such a system will not only greatly increase our in vivo optical imaging capabilities, but also allow integration with data collected by magnetic resonance imaging (MRI) and high resolution micro CT scanning. UCSD has one of the finest state of the art functional- MRI imaging facilities the world, and together these complementary imaging modalities will allow investigators to perform research at a level impossible to achieve before. Signals localized to three- dimensional quantitative anatomical structures will enhance precision in studies ranging from normal development to disease pathologies. Moreover, there are many types of experiments that can be designed to image tissues and cells ex vivo, and utilize this imaging system for high throughput screening. Importantly, this optical imaging facility will be part of a multi-module Physiology Core being developed to provide small animal functional assays, which include cardiovascular dynamics, metabolic analysis, and pulmonary function. Support from these multiple small animal function modules will complement imaging modalities to better understand the physiological bases of collected imaging data. The School of Medicine Medical Teaching Facility community (despite the name this is primarily a research oriented community) is in critical need of a comprehensive resource for incorporating non-invasive imaging data with methods to assess changes in integrative physiological function. All MTF researchers will have access to this state-of-the-art optical imaging data acquisition method, software, as well as training and education for data processing, image analysis, and data integration methods. As development of acquisition and analysis techniques accelerate, the core of investigators incorporating optical imaging into their research programs will provide a knowledge base for investigators that make up the UCSD community, will make these new tools and imaging techniques widely available, and encourage broader use of these technologies. UCSD and the greater San Diego community have strong research programs and this imaging center will add to the ability of researchers to achieve and perhaps even exceed their research goals. Public Health Relevance: Optical imaging is an essential tool for non-invasively studying biological processes in animal models. Understanding and monitoring dynamic changes in physiological systems in both health and disease states will greatly improve the scientific communities ability to diagnose and combat a wide range of illnesses.