Acquisition of laser capture microdissection microscope

描述（由申请人提供）：本提案是徕卡LMD7000激光捕获显微解剖系统的请求。该仪器允许从冷冻或福尔马林固定石蜡包埋的档案组织薄片中分离单细胞或小群细胞，目的是收集RNA， DNA或蛋白质。它使研究者能够从复杂的组织环境中分析特定的细胞群，其应用包括DNA基因分型，RNA转录谱分析，蛋白质组学和信号转导分析。该提案包含来自15位nih支持的科学家的高度临床相关的项目。这些项目包括多项研究，重点关注细胞因子和内皮在脓毒症控制中的作用，hpv诱导的呼吸道乳头状瘤、慢性淋巴细胞白血病、脑肿瘤和其他肿瘤的癌症/增生相关研究，血脑屏障的调节，肺动脉高压，肾小球硬化和酒精成瘾。还包括对B细胞分化的更基本的研究。因此，LCM显微镜的收购将显著提高超过18个nih资助项目的成果。此外，LCM技术应该为范斯坦研究所的长期研究目标做出重大贡献：将对生物机制的理解转化为新的治疗和诊断方法。此外，它将加强和扩大范斯坦研究所与北岸-长岛犹太卫生系统各临床部门之间的现有合作，并最大限度地利用其组织捐赠计划。LCM系统将被安置在范斯坦研究所的光学显微镜核心设施（LMCF）中。仪器的维护、技术指导和调查人员的培训将得到监测监测基金当前管理部门的支持。该申请的PI，同时也是LMCF的主管，与光学显微镜和核心服务咨询委员会一起，将监督LCM系统的使用。北岸大学医院的一名病理学家作为顾问，以及其他一些合作病理学家将进一步支持以lcm为基础的工作。一个财务计划到位，以收回维修成本。美国国立卫生研究院资助的研究人员将优先使用LCM系统，并努力将LCM技术及其应用介绍给新的研究人员。

DESCRIPTION (provided by applicant): This proposal is a request for a Leica LMD7000 laser capture microdissection system. This instrument allows for the isolation of single cells or small groups of cells from thin sections of frozen or formalin-fixed paraffin-embedded archival tissue, with the purpose of collecting RNA, DNA or proteins. It enables the investigator to analyze a specific cell population out of a complex tissue environment, with applications including DNA genotyping, RNA transcript profiling, proteomics and signal transduction analysis. This proposal features highly clinically relevant projects from 15 NIH-supported scientists. These projects include a number of studies that focus on the role of cytokines and the endothelium in the control of sepsis, several cancer/hyperplasia-related studies on HPV-induced respiratory papillomas, chronic lymphocytic leukemia, brain tumors and other tumors, the regulation of the blood-brain barrier, pulmonary hypertension, glomerulosclerosis and alcohol addiction. A more basic study on B cell differentiation is also included. Thus, the acquisition of the LCM microscope will significantly enhance the outcomes of over 18 NIH-funded grants. Moreover, the LCM technology should contribute significantly to the long-range research goal of The Feinstein Institute: the translation of the understanding of biological mechanisms to novel treatments and diagnostics. In addition, it will strengthen and expand existing collaborations between the Feinstein Institute and the various clinical departments of the North Shore-Long Island Jewish Health System and maximize the use of its Tissue Donation Program. The LCM system will be housed in the Light Microscopy Core Facility (LMCF) of the Feinstein Institute. Maintenance of the instrument and technical guidance and training of investigators will be supported by the current management of the LMCF. The PI of this application, who also is the LMCF supervisor, in conjunction with the Light Microscopy and Core Services Advisory Committees, will oversee the utilization of the LCM system. LCM-based efforts will be further supported by a pathologist at the North Shore University Hospital, who serves as a consultant, in addition to a number of other collaborating pathologists. A financial plan is in place to recover maintenance costs. Utilization of the LCM system by NIH-funded investigators will be prioritized and efforts will be made to introduce LCM technology and its application to new investigators.