Olympus FV1000-MPE Confocal & Multi-Photon Imaging System

描述（由申请人提供）：本申请申请资金用于购买奥林巴斯FV1000-MPE共聚焦和多光子荧光显微镜成像系统，专用于小动物活体显微镜。活体显微镜系统将由密歇根大学分子成像中心负责安置、管理和维护，该中心是由美国国立卫生研究院小动物成像资源项目和密歇根大学综合癌症中心资助的核心设施。共聚焦和多光子活体显微镜是一项令人兴奋的技术，用于研究正常生理，多种疾病的动物模型，以及活体动物细胞和亚细胞水平上的治疗效果。密歇根大学拥有最先进的全动物成像设备，但目前在大学或附近的机构没有用于活体动物共聚焦/多光子显微镜的仪器。小动物成像技术的不足限制了研究人员将细胞和分子研究从体外细胞培养系统转化为活体动物生理相关背景的能力。该仪器将被功能和分子成像领域的知名研究人员使用，为癌症生物学、细胞信号传导和诊断显像剂开发等正在进行的项目提供一个重要的工具，以实质性地扩展科学知识和最终临床影响。用于小动物成像的专用共聚焦/多光子仪器的可用性预计将吸引更多的研究人员作为该技术的用户，从而导致新的研究合作，并显着扩大大学的基础和转化研究范围。除了在广泛的健康科学领域取得卓越成就外，密歇根大学还拥有国际公认的光学成像技术和仪器专家，他们致力于支持活体显微镜的技术发展和进步。因此，我们期望活体显微镜系统成为新的多学科研究的焦点，包括光学物理学家、分子生物学家、疾病发病机制和治疗专家以及分子成像。预计这些新的研究合作将导致该大学的拨款增加，从而产生更多的研究工作。相关性：提出的仪器将能够在活的动物细胞和亚细胞水平上研究疾病和对治疗的反应，我们期望这将极大地加速基础科学发现从细胞培养系统到动物模型和最终临床医学的转化。使用该仪器将打开新的研究领域，并在大学的研究人员之间开展合作，从而带来额外的资金和创造新的研究工作。该大学致力于减少能源消耗，并将这一仪器纳入环保工作场所的总体规划中。

DESCRIPTION (provided by applicant): This application requests funds to purchase an Olympus FV1000-MPE confocal and multi-photon fluorescence microscope imaging system dedicated to intravital microscopy in small animals. The intravital microscope system will be housed, managed, and maintained by the Center for Molecular Imaging at University of Michigan, a core facility funded by the NIH Small Animal Imaging Resource Program and the University of Michigan Comprehensive Cancer Center. Confocal and multiphoton intravital microscopy is emerging as an exciting technology to investigate normal physiology, animal models of multiple diseases, and effects of therapy at a cellular and subcellular level in a living animal. University of Michigan has state-of-the art equipment for whole animal imaging modalities, but there currently is no instrument for confocal/multiphoton microscopy of living animals at the university or nearby institutions. This deficiency in small animal imaging technology limits the ability of investigators to translate cellular and molecular studies from in vitro cell culture systems into the physiologically relevant context of a living animal. The proposed instrument will be used by established investigators in functional and molecular imaging, providing a critical tool to substantially expand the scientific knowledge and ultimate clinical impact of ongoing projects in cancer biology, cell signaling, and development of diagnostic imaging agents. Availability of a dedicated confocal/multiphoton instrument for small animal imaging is expected to attract many additional investigators as users of this technology, leading to new research collaborations and markedly expanding the scope of basic and translational research at the university. In addition to excellence across a wide range of health science fields, the University of Michigan has internationally recognized experts in optical imaging technologies and instrumentation who have committed to supporting technical development and advancement of intravital microscopy. Therefore, we expect the intravital microscope system to become a focal point for new multidisciplinary research efforts including optical physicists, molecular biologists, experts in disease pathogenesis and treatment, and molecular imaging. These new research collaborations are anticipated to lead to increased grant funding at the university, resulting in more research jobs. Relevance: The proposed instrument will enable studies of diseases and responses to therapy at the cellular and subcellular level in living animals, which we expect to greatly accelerate translation of basic science discoveries from cell culture systems to animal models and ultimately clinical medicine. Using this instrument will open new research areas and collaborations among investigators at the university, leading to additional funding and creation of new research jobs. The university is committed to reducing energy consumption and will integrate this instrument into the overall plan for an environmentally-friendly workplace.