Qiagen 96-Sample Pyrosequencer

描述（由申请人提供）：设备要求：本申请寻求对购买Biotage/Qiagen公司生产的Pyromark Q96 MD自动焦磷酸测序系统的支持。该系统将允许大量nih支持的研究人员在表观遗传学研究中对DNA甲基化进行高通量定量。原理：焦磷酸测序是一种实时“合成测序”技术，特别适用于DNA甲基化的定量分析。用户组对环境因素及其对健康影响的机制进行研究，包括对与发育、肿瘤和功能变化有关的表观遗传修饰进行比较分析。焦磷酸测序将为研究人员提供高通量和定量分析全局和位点特异性甲基化的能力，精度很高。实施：仪器将位于目前运行的一个完善的核心设施内，并由NIEHS中心拨款支持。仪器将由指导NIEHS核心设施的PI管理，并且在核心设施运营方面具有丰富的用户利益经验。将对两名具有细胞和分子生物学经验的高级技术人员进行系统培训，并为用户组实验室成员提供培训、监督和支持。支持包括核心设施（机构）内的仪器空间，服务合同的覆盖范围和系统的未来组件替换（机构），以及核心设施人员支持（NIEHS中心资助）。一个内部咨询委员会将监督该系统的使用并确保其可访问。摘要：该仪器将通过机构支持、完善的核心设施显著提高大量nih支持的研究人员的研究能力和生产力。

DESCRIPTION (provided by applicant): Equipment Requested: This proposal seeks support for acquisition of a Pyromark Q96 MD automated pyrosequencing system made by Biotage/Qiagen. The system will allow high-throughput quantitation of DNA methylation in epigenetic studies by a large number of NIH-supported researchers. Rationale: Pyrosequencing is a real-time 'sequencing-by-synthesis' technology that is especially useful for quantitative analysis of DNA methylation. The user group conducts studies of environmental agents and the mechanisms of their health effects, including comparative analysis of epigenetic modifications linked to developmental, neoplastic and functional changes. Pyrosequencing will offer the investigators the ability to perform high-throughput and quantitative analyses of global and site-specific methylation with great precision. Implementation: The instrumentation will be situated within a well-established core facility in current operation and supported by a NIEHS Center grant. The instrumentation will be managed by the PI who directs the NIEHS core facilities, and has extensive experience in core facility operation for user benefit. Two senior technologists with experience in cell and molecular biology will be trained on the system and will provide training, supervision and support to the user group lab members. Support includes space for the instrumentation within the core facility (institutional), coverage of service contracts and future component replacements for the system (institutional), and core facility staff support (NIEHS Center grant). An internal advisory committee will supervise use and assure access to the system. Summary: This instrumentation will significantly improve the research capacity and productivity of a large number of NIH-supported investigators via an institutionally supported, well-established core facility.