Animal and Antibody Core

动物和抗体核心的目的是提供动物和抗体的所有组件 计划项目。这包括：1)购买和维护非人类灵长类动物；2)生产、质量 包括MGH Galt-KO猪在内的MGH MHC近交系小型猪的控制和维护；3) 购买和维护SCID和转基因小鼠(项目3和5)，包括屏障住房；和4) 小鼠抗猪免疫球蛋白单抗的制备、表型鉴定及鉴定 恒河猴细胞表面抗原。该中心将为老年人的购买、住房和护理提供支持 非人灵长类动物，这将在所有五个项目中使用。这个核心将提供设施， 生产Galt-KO猪和遗传型近交猪所需的管理和技术专长 用于向所有五个项目运送器官、血液和组织的微型猪。牛群得到了维护， 利用P.I.编写的计算机数据库进行监控，由P.I.负责协调育种、质量 每个项目中动物的控制、分配、运输和利用。大多数大型动物将是 致力于项目1和2，这两个项目主要涉及大型动物异种移植。然而， 项目1和2中来自非人类灵长类动物的血液和组织也将用于研究 项目4和项目5分别介绍了感染性物质的传播和血栓调节。同样，猪也是如此 项目1和2中的动物组织将根据需要提供给项目3、4和5。这一策略是 为了限制动物数量、保护资源和降低成本而采用。核心还将继续 正在进行的努力是生产、表型和生化表征一大批现有的小鼠 单抗(MAb)可与猪和狒狒的间质和淋巴细胞群体反应。这些 单抗将改善重组狒狒受体的猪细胞谱系的特征(项目1 和2)，以及移植的猪胸腺组织(项目1和3)。这些单抗将有助于定义 内皮血栓调节和感染性病原体传播的关键分子相互作用 (项目4和项目5)。此外，与白喉毒素结合的T细胞特异性单抗将用于耗尽 在项目1和项目2中，作为受体调理方案的一部分，狒狒中的淋巴细胞种群。 该计划的每个组成部分的成功将取决于质量、可预测性和可获得性 由这个核心提供的动物和抗体。

The purpose of the Animal and Antibody Core is to provide animals and antibodies to all components of this Program Project. This includes: 1) Purchase and maintenance of non-human primates; 2) Production, quality control and maintenance of MGH MHC inbred miniature swine including the MGH GalT-KO swine; 3) The purchase and maintenance of scid and transgenic mice (Projects 3 and 5) including barrier housing; and 4) Production, phenotyping and characterization of murine monoclonal antibodies reactive with swine and baboon cell surface antigens. The Core will provide support for the purchase, housing and care of the nonhuman primates, which will be utilized in all five projects. This Core will provide the facilities, management and technical expertise necessary to produce GalT-KO swine and genetically typed inbred miniature swine for the delivery of organs, blood and tissues to all five projects. The herd is maintained and monitored with the use of a computer database written by the P.I., who will coordinate the breeding, quality control, allocation, transportation and utilization of animals in each Project. Most of the large animals will be committed to Projects 1 and 2, which deal predominantly with large animal xenotransplantation. However, blood and tissues from the non-human primates in Projects 1 and 2 will also be utilized for the studies of transmission of infectious agents and thromboregulation in Projects 4 and 5, respectively. Likewise, pig tissues from animals in Projects 1 and 2 will be provided to Projects 3, 4 and 5 as required. This strategy was adopted to limit the number of animals, conserve resources, and lower costs. The core will also continue an ongoing effort to produce, phenotype and biochemically characterize a large, existing panel of murine monoclonal antibodies (Mabs) reactive with swine and baboon stromal and lymphocyte populations. These Mabs will allow improved characterization of swine cell lineages reconstituting baboon recipients (Projects 1 and 2), and of engrafted pig thymic tissues (Projects 1 and 3). These Mabs will assist in defining the molecular interactions critical to endothelial thromboregulation and transmission of infectious agents (Projects 4 and 5). In addition, T cell-specific Mabs conjugated to diphtheria toxin will be used to deplete lymphocyte populations in baboons as part of the recipient conditioning regimens in Projects 1 and 2. The success of each component of this program will be dependent on the quality, predictability, and accessibility of the animals and antibodies provided by this Core.