Synthetic Chemical Biology

项目摘要 合成化学生物学核心（SCBC）成立于2016年7月，由中心合并为 疾病途径分子分析（CMADP）分子探针核心（MPC）， 传染病化学生物学（CBID）药物化学核心（MCC）。合成能力 SCBC将实现COBRE两个中心的具体目标。SCBC在第二阶段的使命是提供 隶属于CMADP的初级教师，以及堪萨斯州的其他研究人员， 生物系统的探测器。SCBC还将提供在体外对这些探针进行整体评估的能力- 细胞生物测定和斑马鱼体内试验，斑马鱼是生物医学研究中越来越重要的模式生物。的 SCBC还将履行CBID在抗感染药物发现方面的使命。这些项目将得到资助。 通过CBID。SCBC总监负责协调所有SCBC核心实验室活动， 中心. SCBC将提供分子探针的专家设计和小分子和 肽，重点是荧光和其他标记分子的产生。SCBC还将提供 分子探针的生物测定，包括全细胞测定和使用斑马鱼的测定，以弥补 目前存在于培养细胞的极高分辨率成像和荧光成像的低分辨率成像之间。 啮齿动物体内的示踪剂新成立的专责小组委员会将与其他获“华人社区发展计划”资助的核心小组委员会发挥协同作用 设施，包括基因组测序核心，以确定影响体内探针功能的基因，以及 微加工和微流体核心，用于开发成像和化学分析设备。这 整合旨在使教师能够使用化学生物学方法来研究生物学 问题 SCBC为调查人员提供了接触具有合成有机经验的高素质人员的机会。 化学，生物化学，显微镜，以及优化合成生物学特性的方法 分子。该中心有两名来自KU药物化学系的联合核心领导人。Blake教授 彼得森将继续担任CMADP的SCBC部分的领导人。汤姆Prisinzano是领导者 SCBC的CBID部分。SCBC的主任是Chamani Perera博士。具体目标将是 SCBC在第二阶段提案中提出的问题是：（1）继续整合拟议中的资源， COBRE疾病途径分子分析中心II期与现有的COBRE疾病途径分子分析中心 传染病化学生物学第一阶段进一步建立新的SCBC，获得规模经济， 并为COBRE CMADP的相关人员提供服务;（2）生物分子探针的设计与合成 系统的教师调查员;（3）使教师的客户进行生物测定细胞培养和斑马鱼 （4）提供管理核心活动所需的外联和行政支持， 为堪萨斯州更大的生物医学研究社区提供服务。

PROJECT SUMMARY The Synthetic Chemical Biology Core (SCBC) was established in July 2016 from the merger of the Center for Molecular Analysis of Disease Pathways (CMADP) Molecular Probes Core (MPC) with the newly established Chemical Biology of Infectious Disease (CBID) Medicinal Chemistry Core (MCC). The synthetic capabilities of the SCBC will fulfill the specific aims of both COBRE centers. The mission for the SCBC in Phase II is to provide the junior faculty affiliated with CMADP, as well as other investigators in the state of Kansas, with novel molecular probes of biological systems. The SCBC will also offer the capability to evaluate these probes in vitro in whole- cell bioassays, and in vivo in zebrafish, an increasingly important model organism for biomedical research. The SCBC will also fulfill CBID’s missions concerning anti-infective drug discovery. These projects will be funded through the CBID. The SCBC Director is responsible for coordinating all SCBC Core lab activities to both COBRE centers. The SCBC will offer expert design of molecular probes and synthesis of both small molecules and peptides, with an emphasis on the generation of fluorescent and other tagged molecules. SCBC will also offer bioassays of molecular probes, including whole cell assays and assays using zebrafish, to bridge a gap that currently exists between very high resolution imaging of cells in culture and low resolution imaging of fluorescent tracers in rodents. The newly established SCBC would function synergistically with other CMADP-funded Core facilities, including the Genome Sequencing Core, to identify genes that impact functions of probes in vivo, and the Microfabrication and Microfluidics Core, to develop devices for imaging and chemical analysis. This integration is designed to empower faculty to use chemical biology approaches to investigate biological problems. The SCBC provides investigators with access to highly qualified personnel with experience in synthetic organic chemistry, biochemistry, microscopy, and approaches for optimization of biological properties of synthetic molecules. The Center has two Co-Core Leaders from the Department of Medicinal Chemistry at KU. Prof. Blake Peterson will continue to be the Leader of the CMADP’s part of the SCBC. Prof. Tom Prisinzano is the Leader of the CBID part of the SCBC. The Director of SCBC is Dr. Chamani Perera. The specific aims that will be addressed by the SCBC for this Phase II proposal are: (1) to continue to integrate resources from the proposed COBRE Center for Molecular Analysis of Disease Pathways Phase II with the existing COBRE Center for Chemical Biology of Infectious Disease Phase I to further establish the new SCBC, gain economies of scale, and serve faculty affiliated with the COBRE CMADP; (2) design and synthesize molecular probes of biological systems for faculty investigators; (3) enable faculty clients to conduct bioassays in cell culture and zebrafish models; and (4) provide outreach and administrative support necessary to manage Core activities, and offer services to the larger biomedical research community in the state of Kansas.