Effects of residual PAH exposure from firefighters' turnout gear on plasma microRNA expression

项目总结/摘要 博士Jooyeon Hwang是新加坡国立大学职业与环境健康系助理教授， 俄克拉荷马州健康科学中心。她在致癌物暴露方面有广泛的培训和经验 职业流行病学研究的评估，她希望在 人类微生物组和表观遗传生物标志物的研究。由于表观遗传评估是一种新的方法， 她的领域，她正在申请指导研究科学家发展奖（K 01），以实现三个 培训目标：1）成为一个独立的研究人员在一个新兴的研究领域-保险相关 表观遗传评估，2）将miRNA特征整合到致癌问题的预测中，以及3） 为消防员制定干预和预防策略。为了实现这些目标，黄博士将 接受多学科团队的指导，咨询咨询委员会，完成课程， 培训方案，并参加国家会议和讲习班。她提出的研究地址1） 公共安全部门与癌症、生殖、心血管等慢性病预防 国家职业研究议程（诺拉）的跨部门和2）1.9 C：接触致癌物- 因穿戴受污染的装备而暴露，目标1下的转化研究：减少职业性接触 癌症、心血管疾病、不良生殖结局和其他慢性疾病 2019-2023年战略计划。 在拟议的研究中，Hwang博士将研究可能导致 消防员患皮肤癌的风险增加，包括黑色素瘤和非黑色素瘤，有两个具体目标：1） 评估消防员消防装备和皮肤上PAH残留水平与暴露之间的关系 来自道岔齿轮实践的变量和2）评估皮肤上PAH的浓度和PAH- 相关的microRNA（miRNA）在消防员血液中的表达。拟议研究的结果将 用于增强目前对使用miRNA表达的PAH致癌暴露的理解 分析，描述癌症途径，发展和进展。本研究的贡献将 是双重的。首先，将开发一种新的暴露评估策略， 消防员生物标志物中的基因改变。第二，本研究以职业为基础， 在火灾活动期间暴露之前和之后的PAH相关miRNA的机制将被传播 向地区和全州的消防员以及科学界宣传。黄博士会整理结果 并提交了R 01，重点是信使RNA（mRNA）-miRNA的综合分析， 消防员队伍第二次分析将同时对参与者进行跟踪， 纵向研究

Project Summary/Abstract Dr. Jooyeon Hwang is an Assistant Professor of Occupational and Environmental Health at the University of Oklahoma Health Sciences Center. She has extensive training and experience in carcinogenic exposure assessments for occupational epidemiological studies, knowledge and skills that she hopes to leverage in the study of human microbiomes and epigenetic biomarkers. As epigenetic assessment is a new methodology in her field, she is applying for the Mentored Research Scientist Development Award (K01) to achieve three training goals: 1) to become an independent researcher in an emerging area of study – exposure-associated epigenetic assessment, 2) to integrate miRNA characteristics into the prediction of carcinogenic issues, and 3) to develop intervention and prevention strategies for firefighters. To achieve these goals, Dr. Hwang will receive mentoring from a multidisciplinary team, consult with an advisory committee, complete coursework and training programs, and attend national meetings and workshops. Her proposed research addresses 1) the Public Safety Sector and the Cancer, Reproductive, Cardiovascular and Other Chronic Disease Prevention Cross-Sector of the National Occupational Research Agenda (NORA) and 2) 1.9C: Exposure to carcinogens - Exposures from wearing contaminated gear, Translational Research under Goal 1: Reduce Occupational Cancer, Cardiovascular Disease, Adverse Reproductive Outcomes, and Other Chronic Disease of the Strategic Plan for 2019-2023. The proposed study, in which Dr. Hwang will investigate factors and mechanisms potentially contributing to firefighters' elevated risk of skin cancer, including melanoma and non-melanoma, has two specific aims: 1) to assess associations between residual levels of PAH on firefighters' turnout gear and skin with exposure variables derived from turnout gear practices and 2) to assess the concentration of PAH on skin and PAH- associated microRNA (miRNA) expression in blood of the firefighters. The results of the proposed study will be used to enhance the current understanding of carcinogenic exposure to PAH using miRNA expression profiling, which describes cancer pathways, development, and progression. The contributions of this study will be twofold. First, a novel exposure assessment strategy will be developed that synthesizes the epigenetic alteration of genes in biomarkers of firefighters. Second, this occupation-based study for understanding the mechanisms of the PAH-associated miRNA before and after exposure during fire activities will be disseminated to regional and statewide firefighters as well as the scientific community. Dr. Hwang will compile the results from this study and submit an R01 focusing on an integrated analysis of messenger RNA (mRNA)-miRNA in the firefighter cohort. The second analysis will simultaneously enable a follow-up with the participants for a longitudinal study.