Integrated microbiome analysis of the gut-blood-brain axis to delineate progression to Alzheimer's Disease

我们对健康供者(HC)、轻度认知障碍(MCI)和阿尔茨海默病(AD)所致轻度痴呆(AD)个体血清中的EVS进行了110bp的RNA测序(RNA-seq)。我们观察到AD/MCI/HC血清中EV的线粒体RNA丰度有显著差异。我们用RT-qPCR分析验证了这些变化，并从每组另外5个人身上获得了血浆样本，并进行了RT-qPCR分析。综上所述，我们发现在MCI和AD患者的EVS中，mt-RNAs的水平强劲且明显丰富。我们目前正在进行元基因组测序，并将我们的数据与DTI的结果相关联。

We performed RNA sequencing (RNA-seq) of the EVs in the serum of HC (Healthy donors), MCI (mild cognitive impairment) and AD (mild dementia due to Alzheimers disease) individuals with 110 bp paired-end directional reads. We observed significant differences in mitochondrial RNA abundance in EVs from AD/MCI/HC serum. We validated these changes using RT-qPCR analysis and obtained plasma samples from an additional 5 individuals per group and performed RT-qPCR analysis. In summary, in EVs of MCI and AD patients, we found the levels of mt-RNAs are robustly and significantly more abundant. We are currently working on the metagenomic sequencing and corelating our data with the results of DTI.