Shared Resource 07: Flow Cytometry (FCSR)

项目总结-流式细胞术共享资源（FCSR） 流式细胞术服务对癌症研究至关重要。由于流式细胞术仪器昂贵， 通常需要大量的技术专业知识，因此集中资源至关重要。FCSR的使命是 通过高质量的细胞分析支持OSUCCC成员和高影响力的癌症研究项目， 分类能力。该中心还提供培训，并定期举办技术研讨会或 向OSUCCC成员介绍新技术的研讨会。社会保险基金会继续努力成为一个 最先进的细胞分析实验室，具有以下具体目标：1）提供最先进的设备， 支持OSUCCC成员在收费服务的基础上进行高质量的癌症研究; 2）持续工作 与OSUCCC成员合作，为最先进的细胞仪提供实质性的技术专长和培训 解决癌症研究中基本问题的工具，以便研究人员可以24小时访问 向OSUCCC成员介绍新的仪器、技术和 通过开展各种教育宣传活动，在社会责任委员会制定各种方法。该FCSR公司- 董事是杰弗里查尔默斯，（化学和生物分子工程系），最近任命 联合主任Kevin Weller，Pelotonia免疫肿瘤学研究所（PIIO）副主任， 特别是开发免疫监测和发现平台，这是一个跨领域的共享资源计划 它为复杂的免疫肿瘤学项目整合了共享资源。Gregory Behbehani博士（LR） 是Helios质谱细胞仪（CyTOF）系统的教员顾问。在目前的赠款周期内， 与设立私人投资信息办公室配合，这将导致 需要流式细胞仪服务。为了满足这一需求，购买了新的仪器，包括四个新的 仪器，包括最先进的Cytek Aurora流式细胞仪和Helios质谱细胞仪。在此 在此期间，FCSR发表了231篇出版物（39 >10影响因子），有296名用户，34，118小时 的服务（83.7%的OSUCCC成员），并支持80 NCI赠款（1 K12，1 K22，1 K24，6 P01，1 P50， 52个R 01、1个R 03、7个R21、3个R35、1个R37、1个T32和5个U 01）。在下一个供资周期，FCSR将支持 所有OSUCCC战略优先事项的需求不断增加，包括免疫肿瘤学，转化基因组学， 癌症工程，癌症预防和生存。用户群将大幅增加， 新的工作人员和技术，例如通过购买额外的Aurora仪器，并增加细胞 分选、细胞分离和外泌体/微囊泡研究。此外，正在开发的新服务包括 开发进一步的外泌体表征和分离技术。该基金会的年度预算为： 518,075美元，而CCSG的要求是108261美元。因此，《财务报告准则》利用广泛的机构支持， 只有20.9%来自CCSG基金。

PROJECT SUMMARY – FLOW CYTOMETRY SHARED RESOURCE (FCSR) Flow cytometry services are critical for cancer research. Since flow cytometry instrumentation is expensive, and typically requires significant technical expertise, a centralized resource is essential. The mission of the FCSR is to support OSUCCC members and high-impact cancer research projects with high quality cell analysis and sorting capabilities. The FCSR also provides training and regularly host technology-based seminars or workshops to introduce new technology to OSUCCC members. The FCSR continues to strive to be a state-of- the-art cell analysis laboratory that has the following Specific Aims to: 1) provide state-of-the-art equipment and support for high-quality cancer research for OSUCCC members on a fee-for-service basis; 2) continuously work with OSUCCC members to provide substantial technical expertise and training for state-of the-art cytometry instruments to address fundamental questions in cancer research so that researchers can have 24-hour access to flow cytometry instrumentation; and 3) introduce OSUCCC members to new instrumentation, technology and methodologies being developed at the FCSR through a variety of educational outreach activities. The FCSR co- Directors are Jeffrey Chalmers, (Department of Chemical and Biomolecular Engineering), and recently appointed co-Director, Kevin Weller, Associate Director of the Pelotonia Institute of Immuno-Oncology (PIIO) and specifically to develop the Immune Monitoring and Discovery Platform, a cross-cutting shared-resource initiative that integrates shared resources for complex immuno-oncology projects. Further, Dr. Gregory Behbehani (LR) is a Faculty Advisor for the Helios mass cytometer (CyTOF) system. Over the current grant cycle, major changes to the FCSR were to align with the establishment of the PIIO, which will result in a substantial increase in the need for flow cytometry services. To address this need, new instrumentation was purchased including four new instruments including a state-of-the-art Cytek Aurora flow cytometer and a Helios mass cytometer. During this time period, the FCSR contributed to 231 publications (39 >10 impact factor), had 296 users with 34,118 hours of service (83.7% to OSUCCC members), and supported 80 NCI grants (1 K12, 1 K22, 1 K24, 6 P01s, 1 P50, 52 R01s, 1 R03, 7 R21s, 3 R35s, 1 R37, 1 T32, and 5 U01s). In the next funding cycle, the FCSR will support the increasing needs of all OSUCCC strategic priorities, including immuno-oncology, translational genomics, cancer engineering, and cancer prevention and survivorship. The user base will substantially increased requiring new staff and technologies, for example by purchasing an additional Aurora instrument, and increasing cell sorting, cell isolation, and exosome/microvesicle research. In addition, new services under development include development of further exosome characterization and isolation technology. The annual budget of the FCSR is $518,075, yet the CCSG request is $108,261. As such, the FCSR leverages extensive institutional support and seeks only 20.9% support from CCSG funds.