Supplement application for a CLARIOstar Plus microplate reader with six detection modalities

补充摘要 CLARIOstar Plus六种检测模式微板仪的补充应用 这是购买CLARIOstar Plus多模式微版的行政补充申请 来自BMG LabTech的读者。CLARIOStar是一款高性能、中等吞吐量的仪器， 环境控制为我们的日常使用的六种检测模式提供了统一的解决方案 NIGMS资助的工作：UV/VIS吸光度、荧光、发光、荧光偏振、 时间分辨荧光和α分析。我请求CLARIOStar的原因如下。(1) 它将取代我实验室中用于Hedgehog和WNT的损坏的、已有十年历史的微板阅读器 信号分析是我的NIGMS Mira(R35 GM118082)奖支持的所有项目的核心。(2)它将 推进我在2021年NIGMS R35/Mira续订中概述的研究计划的目标，通过加强我们的 通过环境控制、蛋白质-蛋白质和小分子蛋白质进行活细胞分析的能力 相互作用测量和动力学测量，速度、灵敏度和动态性能都提高了十倍 与我们以前的仪器相比，它的射程更大。(3)具有这些功能的微型平板阅读器目前还没有 考虑到我们密集的使用需求，斯坦福大学以经济高效的方式提供。生物化学系 将用仪器服务的资金和容纳微型板的空间来支持这次购买 读者。总之，CLARIOstar将加速我实验室和NIGMS资助的研究项目 为从事这些项目的学员提供测试设计和优化方面的尖端培训。

Supplement Summary Supplement application for a CLARIOstar Plus microplate reader with six detection modalities This is an administrative supplement application for the purchase of a CLARIOstar Plus multi-mode microplate reader from BMG Labtech. The CLARIOStar is a high-performance, medium-throughput instrument with environmental control that provides a unifying solution to the six detection modalities used routinely in our NIGMS-funded work: UV/Vis absorbance, fluorescence, luminescence, fluorescence polarization, time-resolved fluorescence and ALPHA assays. I am requesting the CLARIOStar for the following reasons. (1) It will replace a broken, decade-old microplate reader that is used in my laboratory for Hedgehog and WNT signaling assays central to all the projects supported by my NIGMS MIRA (R35 GM118082) award. (2) It will advance the goals of the research program outlined in my 2021 NIGMS R35/MIRA renewal by enhancing our capability to perform live cell assays with environmental control, protein-protein and small molecule-protein interaction measurements, and kinetic measurements, all with ten-fold higher speed, sensitivity and dynamic range compared to our previous instrument. (3) A microplate reader with these capabilities is not presently available at Stanford on a cost-effective basis given our intensive usage needs. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the microplate reader. In summary, the CLARIOstar will accelerate NIGMS-funded research projects in my laboratory and provide cutting-edge training in assay design and optimization for trainees that work on these projects.