Caenorhabditis Genetics Center

项目摘要/摘要--总体 线虫遗传中心(CGC)是唯一一个全面的储存库和分发中心 线虫秀丽线虫，生物医学研究的首选模式生物。整体而言 该动物资源的目标是通过获取、维护和分发线虫来促进线虫的研究 具有基因特征的线虫种群。所有50个州和世界各地的研究人员使用基因 从CGC获得的各种基础和应用研究工作以及实践教学的库存 实验科学的发展。对线虫的研究使人们对基本生物学有了基本的了解 机制，包括细胞程序性死亡的遗传基础，microRNA的发现，以及 动物体内的RNA干扰机制。线虫还提供了对细胞信号转导的关键见解 机制、癌症进展和神经退行性疾病。此外，线虫还是一把钥匙 阐明我们对与人类和牲畜健康相关的寄生线虫的理解的模型。 作为线虫唯一的总储备中心，CGC是一项极其重要的国际研究 资源，支持这些不同领域的研究和教育努力。CGC提供了以下内容 每年向数千个实验室发送30,000个菌株；收集了23,000个独特的菌株，一个数字仍然 随着该领域的发展，CGC不仅促进了研究，还确保了 有价值的菌株被保存下来。应要求，菌株通过在线订购系统分发，并带有 制定用户收费计划，以帮助支付成本和支持CGC活动。CGC密切监控用户 需要并设计小型研究项目来支持社区。因为基本上每个实验室都使用 荧光蛋白，以及对更明亮、更耐光的荧光蛋白的需求，在更多的颜色 允许多路复用，CGC将开发一条管道来定量评估一组新的体内特性 通过对蠕虫中荧光蛋白的研究，科学家能够为它们的应用选择最佳的版本。

Project Summary/Abstract—Overall The Caenorhabditis Genetics Center (CGC) is the sole comprehensive repository and distribution center for the nematode Caenorhabditis elegans, a premier model organism for biomedical research studies. The overall objective of this animal resource is to promote research on C. elegans by acquiring, maintaining, and distributing genetically characterized nematode stocks. Researchers in all 50 states and throughout the world use genetic stocks obtained from the CGC in diverse basic and applied research endeavors, as well as for hands-on teaching of experimental science. Studies using C. elegans have led to fundamental insights into basic biological mechanisms, including the genetic basis of programmed cell death, the discovery of microRNAs, and the mechanism of RNA interference in animals. The nematode has also provided key insights into the cell signaling mechanisms, cancer progression, and neurodegenerative diseases. In addition, C. elegans serves as a key model for illuminating our understanding of parasitic nematodes with relevance to human and livestock health. As the only general stock center for C. elegans, the CGC is an extremely important international research resource, supporting research in these diverse areas and in educational endeavors. The CGC provides about 30,000 strains per year to thousands of laboratories; with a collection of 23,000 unique strains, a number still increasing in proportion to the growth of the field, the CGC not only facilitates research, but also ensures that valuable strains are preserved. Strains are distributed upon request through an on-line ordering system with a scheme of user fees in place to help defray costs and support CGC activities. The CGC closely monitors user needs and devises small research projects to support the community. Because essentially every lab uses fluorescent proteins, and there is demand for brighter, more photostable fluorescent proteins, in more colors to allow multi-plexing, the CGC will develop a pipeline to quantitatively evaluate the in vivo properties of a new set of fluorescent proteins in worm, enabling scientists to choose the optimal versions for their applications.