JHU Center for the Advancement of HIV Neurotherapeutics (JHU CAHN) - Biomarker Core

项目总结-生物标志物核心 JHU艾滋病神经治疗促进中心（JHU CAHN）将专注于CNS并发症 PWH通常基于NIMH研究领域标准（RDoC）的框架， 根据可观察到的行为和神经生物学测量的维度对CNS并发症进行分类， 包括负价和正价体系（例如，快感缺乏，恐惧），感觉运动系统，系统 社会过程（例如，社交回避），唤醒/调节系统（例如，睡眠）和认知。这 框架提供了新的机会，更准确地将生物标志物与特定生物学相关联 其目的是确定分子靶点，用于通过药物治疗开发个性化药物方法。 治疗和临床核心。生物标志物核心将与治疗和临床核心密切合作 整合多维数据和工具，如机器学习算法，并提供这些服务， 对神经艾滋病病毒感兴趣的研究人员技术服务将包括代谢物的质谱分析， 脂质和蛋白质，多重Meso Scale发现测定，细胞外囊泡分离和货物 特征化提供的信息学分析将包括小分子途径数据库，京都 基因和基因组百科全书，细胞景观和免疫途径分析。高级非参数 分析将包括各种多变量建模，诸如主成分分析（PCA）、偏最小方差分析（PLS）、最小方差分析（LLS）等。 平方判别分析（PLS-DA）和稀疏PLS-DA分析，代谢产物的显著性分析 (SAM)和代谢物经验贝叶斯分析（EBAM）模型，以确定区分 临床生物型或实验组。这些服务将广泛传达给神经艾滋病毒 社区通过我们的多中心每周研讨会系列。这些服务是JHU CAHN独有的， 为NeuroHIV研究人员在职业发展的各个阶段提供宝贵的资源。

PROJECT SUMMARY - BIOMARKER CORE The JHU Center for the Advancement of HIV Neurotherapeutics (JHU CAHN) will focus on a CNS complications that commonly present in PWH based on the framework of the NIMH Research Domain Criteria (RDoC) that classifies CNS complications based on dimensions of observable behavior and neurobiological measures that include Negative and Positive Valence Systems (e.g., anhedonia, fear), Sensorimotor Systems, Systems for Social Processes (e.g., social avoidance), Arousal/Regulatory Systems (e.g., sleep), and cognition. This framework provides new opportunities to more accurately associate biomarkers with particular symptomology with the goal of identifying molecular targets for the development of personalized medicine approaches by the Therapeutics and Clinical Cores. The Biomarker Core will work closely with the Therapeutic and Clinical cores to integrate multi-dimensional data and tools such as machine learning algorithms and offer these services to investigators interested in NeuroHIV. Technical services will include mass spectrometry analysis for metabolites, lipids, and proteins, multiplex Meso Scale discovery assays, extracellular vesicle isolation and cargo characterization. Informatics analyses offered will include Small Molecules Pathway Database, Kyoto Encyclopedia of Genes and Genomes, Cytoscape, and Ingenuity Pathway Analysis. Advanced non-parametric analyses will include a variety of multivariate modeling such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and sparse PLS-DA analysis, Significance analysis of metabolites (SAM), and Empirical Bayesian analysis of metabolites (EBAM) models to identify features that discriminate clinical biotypes or experimental groups. These services will be communicated broadly to the NeuroHIV community through our multicenter weekly seminar series. These services are unique to the JHU CAHN, and provide a valuable resource to NeuroHIV investigators at all stages of career development.