Core B Ocular Imaging Core

摘要 眼部成像核心 眼科成像核心将为参与P30 Cole Eye的研究人员提供各种成像方式 视觉研究核心。该模块包括带裂隙灯的活体动物成像和眼底摄影， 扫描激光眼底镜(SLO)和光学相干断层扫描(OCT)。它还支持组织和细胞 成像(体外)支持，提供常规和专门组织固定技术的培训和协助， 石蜡和塑料组织包埋、切片(石蜡和冷冻)，免疫组织化学染色。 显微镜成像技术包括明视场、暗视场、差分干涉对比(DIC)、荧光 和共聚焦显微镜。它还提供有关所生成图像的形态分析的帮助和建议。访问 这个核心模块将支持NEI R01资助的调查人员，帮助新招聘的调查人员获得NEI的数据 R01拨款申请，促进RO1资助的调查人员和新招聘人员之间的协作互动 研究人员，吸引其他部门的研究人员来研究视觉系统，并成为有价值的 部门资源，以吸引更多的视觉科学家加入我们的项目，因为我们扩大了我们的研究人员队伍。

Abstract Ocular Imaging Core The Ocular Imaging Core will provide a variety of imaging modalities to the investigators involved in this P30 Cole Eye Vision Research Core. The module includes live animal (in vivo) imaging with slit lamp and fundus photography, scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT). It also supports tissue and cellular imaging (ex vivo) support, providing training and assistance with routine and specialized tissue fixation techniques, paraffin and plastic tissue embedding, sectioning (paraffin and Cryo) and staining for immunohistochemistry. Microscope imaging techniques include bright field, dark field, differential interference contrast (DIC), fluorescence, and confocal microscopy . It also provides help and advice with the morphometric analysis of generated images. Access to this core module will support NEI R01 funded investigators, help newly recruited investigators obtain data for NEI R01 grant submissions, foster collaborative interactions between RO1-funded investigators and newly recruited investigators, attract investigators from other departments to research on the visual system and be a valuable departmental resource in attracting additional vision scientists to our program as we expand our research faculty.