Host Microbe Core

摘要 宿主微生物核心（HMC）将协助和支持研究基地，学员和试点项目 研究者通过提供标本、试剂、培训和服务来确定微生物和宿主 NIDDK优先考虑的器官和组织中CF疾病的反应决定因素。此核心结合了 CF基础和临床微生物学、气道上皮和免疫细胞专家的资源和专业知识 生物学，病理学和显微镜，以提供一个综合的服务网络，反映了在 CF疾病发病机制的领域，同时调查主机和病原体。提供的核心服务 UW CF P30研究基地包括： 1.组织病理学、免疫组织化学、原位技术援助和先进设施 宿主和微生物靶标的杂交和定位，以及数字成像 2.人胰腺和肺的定量数字病理学和全切片数字库 与CF 3.处理临床、动物和实验室标本，用于微生物组、蛋白质组、代谢组和 转录组分析，并针对特定靶点 4.以代表CF粘膜条件的形式对CF标本进行微生物培养， 蛋白质组学、基因组学和生化分析 5.获得从CF临床粪便样本中培养的细菌菌株，包括大肠杆菌和 拟杆菌属 6.核心小组提供的方法和实验设计方面的咨询和培训 7.与临床和基因组学核心紧密互动，为CF研究人员提供统一服务

ABSTRACT The Host Microbe Core (HMC) will assist and support the research base, trainees, and pilot project investigators by providing specimens, reagents, training, and services to define both the microbial and host response determinants of CF diseases in organs and tissues prioritized by the NIDDK. This Core combines the resources and expertise of specialists in CF basic and clinical microbiology, airway epithelial and immune cell biology, pathology, and microscopy to provide an integrated network of services, reflecting an evolution in the field of CF disease pathogenesis to concurrently investigate the host and pathogens. Core services available to the UW CF P30 research base include: 1. Technical assistance and state-of-the-art facilities for histopathology, immunohistochemistry, in situ hybridization and localization of host and microbial targets, and digital imaging 2. Quantitative digital pathology and whole slide digital repository for pancreata and lungs from people with CF 3. Processing clinical, animal, and laboratory specimens for microbiome, proteome, metabolome, and transcriptome analyses, and for specific targets 4. Microbial culturing of CF specimens in formats representative of CF mucosal conditions for phenotypic, proteomic, genomic, and biochemical analyses 5. Access to strains of bacteria cultured from CF clinical fecal samples, including Escherichia coli and Bacteroides spp. 6. Consultation and training in methods provided by the Core and experimental design 7. Interacting closely with the Clinical and Genomics cores to provide unified services for CF researchers