Animal Metabolism Phenotyping Core

项目总结-动物新陈代谢表型分析核心 动物代谢表型鉴定核心是一家高通量的设备，致力于研究 与肥胖和其他营养相关疾病有关的能量平衡。核心提供对状态的访问- 最先进的方法、仪器和对小鼠种群的访问(如Collaborative Cross Line和 几种UNC特有的基因工程小鼠模型)以确保高水平的质量控制， 重复性和最大吞吐量是个别研究人员无法达到(或负担得起)的。这个 Core提供各种全面、高吞吐量的服务，这些服务可以与营养(和/或 其他环境)处理，例如长期喂养不同的饲料或短期提供特定的 营养素和治疗性化合物。核心为调查人员提供了关于选择的关键信息 适当的饮食，包括使用严格的控制，以及获得合作的交叉和多样性 培育出“下一代”小鼠资源，用于营养和肥胖研究。该中心还提供培训 以及专业发展，因为它涉及研究小鼠能量平衡的组成部分。基于强大的 应UNC NORC成员的需求，核心实施了对 活细胞和分离的线粒体的线粒体表型，这是Core Co. 董事们。 核心服务从北卡罗来纳大学(UNC)教堂山校区扩展到包括 位于北卡罗来纳州坎纳波利斯的北卡罗来纳大学营养研究所(NRI)的额外场地。核心现在有三个 镜像设施。其中两个设施位于科尔大厅的教堂山校区和吉恩 医药大楼。第三个设施在NRI。NRI拥有来自北卡罗来纳大学的基础科学家和翻译科学家 以及依赖鼠标模型进行符合北卡罗来纳大学目标的研究的其他机构/行业 北大西洋公约组织。这种扩张的目的有三个方面。首先，它进一步使NRI的研究人员能够 利用北卡罗来纳大学NORC核心服务。其次，它利用了NRI可用的广泛资源， 包括额外的空间。第三，它使核心服务能够提供给不同的 外部用户，包括阿巴拉契亚州立大学、北卡罗来纳州农工大学、北卡罗来纳州中央大学的学术合作伙伴 北卡罗来纳州立大学、北卡罗来纳州立大学、北卡罗来纳州立大学格林斯伯勒分校和行业合作伙伴。

Project Summary – Animal Metabolism Phenotyping Core The Animal Metabolism Phenotyping Core is a high throughput facility dedicated to studying components of energy balance related to obesity and other nutritionally related diseases. The Core provides access to state- of-the-art methods, instrumentation, and access to mouse populations (such as Collaborative Cross lines and several genetically engineered mouse models unique to UNC) to assure a high level of quality control, reproducibility and maximal throughput that could not be achieved (or afforded) by individual investigators. The Core offers a variety of comprehensive, high-throughput services that can be interfaced with nutritional (and/or other environmental) treatments, such as long-term feeding of different diets or short-term provision of specific nutrients and therapeutic compounds. The Core provides investigators with critical information on the selection of appropriate diets, including the use of rigorous controls, and access to the Collaborative Cross and Diversity Outbred “next generation” mouse resource for nutrition and obesity research. The Core also provides training and professional development as it relates to studying components of murine energy balance. Based on strong demand from UNC NORC members, the Core implemented the scientific and technical support for mitochondria phenotyping of live cells and isolated mitochondria, an area of expertise for the Core Co- Directors. Core services were expanded from the University of North Carolina (UNC) Chapel Hill campus to include an additional site at the UNC Nutrition Research Institute (NRI) in Kannapolis, NC. The Core now has three mirrored facilities. Two of these facilities are located at the Chapel Hill campus in Kerr Hall and in the Genetic Medicine Building. The third facility is at the NRI. The NRI houses basic and translational scientists from UNC and other institutions/industry that rely on mouse models for studies consistent with the objectives of the UNC NORC. The purpose of this expansion was three-fold. First, it further enabled researchers housed at the NRI to utilize the UNC NORC core services. Second, it leveraged the extensive resources available at the NRI, including additional vivarium space. And third, it enables core services to be provided to a diverse group of external users, including academic partners from Appalachian State University, NC A&T University, NC Central University, NC State University, UNC Charlotte, UNC Greensboro, and industry partners.