Multilayer Scaffold for Condyle Surface Regeneration

项目总结 最严重的TMJ疾病包括下颌骨髁状突变性。很遗憾，我不会 再生选择是存在的，目前的治疗方法不能完全恢复功能。指骨的关节组织 髁突是一种纤维软骨，由纤维、软骨和骨之间错综复杂的界面组成。 对正常功能至关重要的组织，在严重的TMJ疾病中丢失。这项研究的目的是 是在骨骼成熟的山羊体内再生下颌骨的纤维软骨-骨界面 综合组织工程学方法。我假设多层脚手架将允许特定的地点 同种异体下颌骨软骨纤维-软骨-骨再生的对比研究 海绵支架和未经处理的对照缺陷。首先，我将研究多层脚手架设计的特性 试管内。我将表征转化生长因子βS从支架中的渗透性和释放性。第二，我会研究 三种细胞亚群在髁突上的再生潜力。第三，我会把我们的脚手架 目的：建立山羊下颌骨髁状突缺损区模型，并评价其功能再生潜能。这项建议 强调了PI将在整个培训期间作出的贡献，这将 主要致力于完成脚手架的制作和表征，以及研究 TMJ细胞亚群的体外再生能力和我们的双层支架的体内再生能力。 这项提案的成功完成是为治疗TMJ提供再生疗法的关键一步。 下颌软骨退行性变，为其他部位成功的骨软骨组织再生奠定了基础。

PROJECT SUMMARY The most severe cases of TMJ disorders consist of mandibular condyle degeneration. Unfortunately, no regenerative options exist, and current treatments do not restore full function. The articulating tissue of the condyle is a fibrocartilage that consists of an intricate interface between fibrous, cartilaginous, and boney tissue that is essential for normal function and that is lost in severe TMJ disorders. The objective of this study is to regenerate fibrocartilage-bone interface of the mandibular condyle in skeletally mature goats using a comprehensive tissue engineering approach. I hypothesize that a multilayer scaffold will allow for site-specific fibrous-cartilage-bone regeneration of the mandibular condyle cartilage when compared to a homogenous sponge scaffold and untreated control defects. First, I will study the properties of a multilayer scaffold design in-vitro. I will characterize the permeability and release of TGFβs from the scaffold. Second, I will study the regeneration potential of three cell subpopulations found on the condyle. Third, I will implant our scaffold in mandibular condyle defects of goats and assess their potential for functional regeneration. This proposal highlights the contributions that the PI will make throughout the duration of the training, which will primarily focus on the completion of scaffold fabrication and characterization, as well as the study of the regenerative potential of TMJ cells subpopulations in vitro and of our bilayer scaffold in vivo. Successful completion of this proposal is the critical step to providing a regenerative therapy to treat TMJ mandibular cartilage degeneration and a basis for successful osteochondral tissue regeneration in other sites.