8-Prenylnaringenin: A Natural Therapy for Estrogen Deficiency

项目总结 所有女性都会经历更年期，这会使女性面临许多慢性病的风险，包括 肥胖风险增加。尽管绝经后雌激素的替代疗法(激素替代疗法-- HRT)已被证明有效地预防与代谢和炎症有关的扰动 由于雌激素缺乏，HRT已被证明会带来不必要的健康风险，包括增加中风的风险， 心脏病发作，以及多种癌症。因此，它已成为国家补充中心的优先事项 和综合健康，投资于开发既安全又有效的治疗方法 更年期引起的副作用，包括过量脂肪堆积及其相关的负面代谢和 炎症性扰动。母公司K01项目(K01AT010348)旨在检查治疗方法 从狼疮属植物(HOPPS)中提取的有效植物雌激素8-戊烯基柚皮苷(8-PN)的潜力。这个 长期目标是开发一种安全有效的自然疗法来治疗更年期的临床-- 相关的代谢损伤和炎症驱动的健康扰动。对于此管理 作为补充，我们建议研究8-PN的不同给药模式对其生物利用度的影响。 以及阐明8-PN影响巨噬细胞表型的机制(S)。 赖利·埃诺斯博士实验室的美洲原住民遗产博士生克里斯蒂安·昂格尔将担任小学学生 这一补充项目的研究人员。在伊诺斯博士的指导下，家长格兰特的少年派克里斯蒂安 将接受质谱学、营养食品生物利用度评估、大数据分析以及 体外细胞培养模型。本补编中提出的研究完全在研究范围之内。 在母公司K01奖中获得支持。除了克里斯蒂安将从中获得的研究经验外 作为补充，克里斯蒂安还将接受科学写作、演示技能、数据分析和 解释、补充和替代医学、免疫学和负责任的研究行为。这 补充剂将为克里斯蒂安成为科学家的长期目标提供垫脚石 研究治疗肥胖症的营养疗法。

PROJECT SUMMARY All women experience menopause, putting a female at risk for a number of chronic diseases, including an increased risk of obesity. Although post-menopausal replacement of estrogen (hormone replacement therapy - HRT) has been shown to be effective at protecting against the metabolic and inflammatory perturbations linked to estrogen-deficiency, HRT has been shown to pose unwanted health risks including an increased risk of stroke, heart attack, and a variety of cancers. As such, it has become a priority of the National Center for Complementary and Integrative Health to invest in the development of therapies that are both safe AND effective at combating menopause-induced side effects, including excess fat mass accrual and its related negative metabolic and inflammatory perturbations. The parent K01 project (K01AT010348) is aimed at examining the therapeutic potential of the potent phytoestrogen from the Humulus Lupus plant (hops), 8-prenylnaringenin (8-PN). The long-term goal is to develop a safe and effective natural therapy for the clinical treatment of menopause- associated metabolic impairments and inflammatory-driven health perturbations. For this administrative supplement, we propose to examine how different modes of administration of 8-PN impact bioavailability of this potent phytoestrogen as well as elucidate the mechanism(s) by which 8-PN impacts the macrophage phenotype. Christian Unger, a PhD student of Native American heritage in Dr. Reilly Enos’ lab, will serve as the primary researcher in this supplementary project. Under the mentorship of Dr. Enos, the PI of the parent grant, Christian will be exposed to mass spectrometry, nutraceutical bioavailability assessment, large data analysis, as well as in vitro cell culture models. The research proposed in this supplement falls well within the scope of the research supported in the parent K01 award. In addition to the research experience Christian will garner from this supplement, Christian will also receive training in scientific writing, presentation skills, data analysis and interpretation, complementary and alternative medicine, immunology, and responsible conduct of research. This supplement will provide Christian with a stepping stone towards his long term goal of becoming a scientist examining nutraceutical therapies for the treatment of obesity.