Immunobiology Core

基因工程猪器官移植到狒狒体内： 免疫学和功能研究 CORE B：免疫生物学CORE（核心负责人：Hidetaka Hara MD，PhD） 总结/摘要 免疫生物学核心（核心B），其中有许多先进的工具，将提供一个 通过执行所有要求的免疫测定，为所有3个项目（肾脏、肝脏、心脏）提供服务， 每项研究。核心B将标准化将在两者之间进行的所有测定 中心（UAB和UMB）这样，在肾脏、肝脏和心脏模型中的研究结果 可以在所用方法没有任何差异的情况下进行比较。 核心将监测移植受者的免疫状态，并试图 确定异种移植物的接受或排斥机制。核心B将应用许多 分子和细胞免疫分析工具，以研究先天和适应性 对基因工程猪移植物的免疫反应。具体而言，我们将评估 器官源猪和外源基因工程的贡献 免疫抑制疗法，以克服异种移植物的免疫屏障，目的是 提供了对异种移植物接受或排斥机制的深入了解。 核心B将协调其与受试者免疫状态相关的发现， 异种移植物与Core C提供的移植物组织病理学相关的那些。我们 假设对全身免疫反应和局部特征的研究 (in移植物）与免疫应答相关，将有助于确定 异种移植损伤和局部免疫调节（例如，外周耐受性）。

GENETICALLY-ENGINEERED PIG ORGAN TRANSPLANTATION IN BABOONS: IMMUNOLOGICAL AND FUNCTIONAL STUDIES CORE B: IMMUNOBIOLOGY CORE (Core Lead: Hidetaka Hara MD, PhD) SUMMARY/ABSTRACT The Immunobiology Core (Core B), which has many advanced tools, will provide a service to all 3 Projects (kidney, liver, heart) by carrying out all of the immunoassays required in each study. Core B will standardize all of the assays that will be carried out between the two centers (UAB and UMB). In this way, the results of studies in the kidney, liver, and heart models can be compared in the absence of any difference in the methods used. The Core will monitor the immune status of the transplant recipients and will attempt to determine the mechanisms of acceptance or rejection of the xenografts. Core B will apply many tools of molecular and cellular immunological assays to investigate both the innate and adaptive immune responses to the genetically-engineered pig grafts. Specifically, we will assess the contributions of the genetic engineering of the organ-source pigs and the exogenous immunosuppressive therapy to overcome the immune barriers to a xenograft, with the aim of providing insight into the mechanisms of xenograft acceptance or rejection. Core B will coordinate its findings relating to the immune status of the recipients of the xenografts with those relating to the histopathology of the grafts provided by Core C. We hypothesize that the investigation of the systemic immune response, and of the local features (in the graft) associated with that immune response, will help determine the mechanism of xenograft injury and of local immune regulation (e.g., peripheral tolerance).