Biospecimen Bank

Core C旨在满足项目的特定需求，同时利用 现有的癌症中心（CC）的核心，没有不必要的重复服务。该核心将密切合作， 与许多CC核心以及其他SPORE核心协同，为所有 建议的项目和可能的SPORE间合作。 这个核心的首要目标是提供高质量，注释良好和严格的质量控制 项目的生物标本，开发和实施生物标本分析所需的测定 并与核心B合作，建立信息学基础设施，以促进研究的各个方面 到生物标本。 具体目标1是在以下领域提供服务：（1）新鲜和石蜡的收购和银行业务- 淋巴瘤的固定组织以及前瞻性获得的血液和骨髓（BM）样本， 回顾性地从COH淋巴瘤SPORE招募的患者中;我们将确保快速收集和 处理、适当的储存和监测，以及适当的注释和记录保存;（2）全面 淋巴瘤标本的检查，以确保正确的诊断和分类，包括 免疫组化、流式细胞术、分子病理学和细胞遗传学研究;（3）性能和 协助淋巴瘤组织和细胞的常规组织学和免疫组化染色，以及 专门的组织学服务，如制备多肿瘤块或组织微阵列， 研究人员的规格。将根据需要提供额外的专门服务。 收集白细胞分离标本以生成CAR-T细胞的项目（项目1）; 通过多光谱多重免疫荧光检测复杂型霍奇金淋巴瘤和宿主细胞群 (mIF)表型和基因表达谱（GEP）（项目3）;细胞遗传学和基因组表征 和制备样品以产生原发性患者来源的异种移植物（PDX）非肿瘤细胞。 霍奇金淋巴瘤模型（项目4）。 由于与核心C功能相关的数据集的复杂性，在具体目标2中，我们将 与核心B（研究信息学）合作，设计、构建和集成与以下内容相关的数据库： 生物标本，以改善其功能和连接，以支持孢子项目和合作， NCI赞助的其他项目。

Core C is designed to meet the specific needs of the projects and at the same time draw on the strength of the existing Cancer Center (CC) cores without unnecessary duplication of services. This Core will work closely and synergistically with a number of CC Cores as well as other SPORE Cores to provide exceptional support for all the proposed projects and possible inter-SPORE collaborations. The overarching goals of this core is to provide high quality, well annotated and strictly quality controlled biospecimens for the projects, to develop and implement assays needed for the analysis of the biospecimens and to collaborate with Core B to build the informatics infrastructures to facilitate all aspects of research linked to biospecimens. Specific Aim 1 is to provides services in the following areas: (1) acquisition and banking of fresh and paraffin- fixed tissues of lymphoma and blood and bone marrow (BM) samples obtained prospectively and retrospectively from patients enrolled on the COH lymphoma SPORE; we will ensure rapid collection and processing, proper storage and monitoring, and proper annotation and record keeping; (2) comprehensive work-up of lymphoma specimens to ensure correct diagnosis and classification, including immunohistochemical, flow cytometric, molecular pathologic, and cytogenetic studies; (3) performance and assistance in routine histologic and immunohistochemical staining of lymphoma tissues and cells, as well as specialized histologic services such as preparation of multi-tumor blocks or tissue microarrays to the specifications of researchers. Additional specialized services will be performed according to the needs of the projects with the collection of leukapheresis specimens to generate CAR-T cells (Project 1); characterization of complex Hodgkin lymphoma and host cell populations through multispectral multiplexed immunofluorescence (mIF) phenotyping and gene expression profiling (GEP) (Project 3); cytogenetic and genomic characterization of lymphomas and the preparation of samples to generate primary patient-derived xenograft (PDX) non- Hodgkin lymphoma models (Project 4). Because of the complexity of the datasets associated with the functions of Core C, in Specific Aim 2, we will collaborate with Core B (Research Informatics) to design, build, and integrate databases relevant to biospecimens to improve their functionality and connectivity to support SPORE projects and collaborations on other NCI-sponsored projects.