A SARS-CoV-2 NFC ePAD Biosensor

本文提出了一种改进的电化学传感器的发展，用于检测 SARS-CoV-2核衣壳蛋白的热塑性电极（TPE）。需要更快 COVID-19大流行期间的检测为RT-qPCR检测带来了几种替代方案， 病毒感染然而，目前的快速测试缺乏灵敏度（侧流测定和免疫分析）。 其它基于纸的测试）或昂贵（基于金的电化学测试）。TPE解决了 敏感性和成本问题，并已被证明比模板印刷碳更强大 电极（SPCE）。TPE表面将使用以下物质的电还原进行共价改性： 芳基重氮盐，然后“点击化学”，以提供一个平台，简单的抗体 交联反应功能齐全的传感器将成为 未来的工作将其整合到一个完整的芯片上分析的微流体装置。

Proposed here is the development of an improved electrochemical sensor for detection of the SARS-CoV-2 nucleocapsid protein using thermoplastic electrodes (TPEs). The need for faster testing amid the COVID-19 pandemic brought about several alternatives to RT-qPCR testing for viral infections. However, current rapid tests are lacking in sensitivity (lateral flow assays and other paper based tests) or expensive (gold based electrochemical tests). TPEs solve both the sensitivity and cost issues and have been shown to be more robust than stencil printed carbon electrodes (SPCE). The TPE surfaces will be covalently modified using electroreduction of aryldiazonium salts followed by “click chemistry” to provide a platform for simple antibody crosslinking reactions. The fully characterized and functional sensor will then be the basis for future work integrating it into a microfluidic device for a full on-chip assay.