B Cell/T Cell Interactions in Brucellosis

项目摘要/摘要 人类布鲁氏菌属(Brucella spp.)会导致终生衰弱的疾病，并反复出现波状发烧和 即使使用抗生素治疗也会出现其他并发症。目前还没有获得许可的疫苗来预防人类 布鲁氏菌病。在这本聚焦补充材料中，我们将使用加州大学的BSL3细胞分选能力 密苏里州区域生物遏制实验室(MURBL)与RNA-SEQ一起执行详细的转录 我们的布鲁氏菌疫苗免疫模型中B和T细胞的分析。我们对此的发现 补充内容将补充我们父母提案的结果，并为我们提供以下附加信息 将加深我们对B细胞抗原提呈如何抑制疫苗介导的免疫的理解 布鲁氏菌，这反过来又可以改进布鲁氏菌病疫苗的合理设计。

Project Summary/Abstract In humans, Brucella spp. can cause a lifelong, debilitating disease, with relapses of undulating fever and other complications even with antibiotic treatment. No vaccines are currently licensed to prevent human brucellosis. In this focused Supplement, we will employ the BSL3 cell sorting capability of the University of Missouri Regional Biocontainment Laboratory (MURBL) along with RNA-seq to perform detailed transcriptional analyses of B and T cells in our model of vaccine-mediated immunity to Brucella. Our findings from this Supplement will complement the results from our parental proposal and provide us additional information which will enhance our understanding of how B cell antigen presentation inhibits vaccine-mediated immunity against Brucella, which in turn could improve the rational design of vaccines for brucellosis.