Physiology Core

摘要 生理学核心提供了一系列的分级技术和专业知识的研究人员谁希望， 解决有关发生在肾单位管状结构中的运输过程的基本问题 及其他相关制度。为了了解介导肾功能的生物学过程， 健康和疾病状态，鉴于该器官的表型多样性和功能复杂性， 允许特定肾单位片段/细胞/蛋白质在定义的条件下分离研究的策略 条件是必需的。核心通过其三个子核心提供专业知识和最先进的技术 用于研究转运和其他膜驻留蛋白的功能和调节， 从单个分子到模型系统再到天然上皮细胞的复杂程度。单管和 微灌注Subcore提供了研究转运功能和表达的工具和技术 从小型哺乳动物分离的单个肾单位节段中的蛋白质和相关调节途径， 肾类器官电生理学子核心提供电生理学的专业知识和工具箱 在分子和细胞水平上研究转运蛋白的功能和调节的方法， 模型系统和天然上皮细胞。活细胞成像子核心提供成像工具和技术， 评估离子转运、膜电位变化、细胞体积、细胞内介质和信号传导 使用化学和遗传编码的荧光标记在永生化细胞和天然制剂中的分子 传感器.核心将采用多管齐下的方法，并与奥布莱恩财团合作， 整个肾脏研究界的专业资源和技术，以降低 进入早期阶段的调查人员和研究人员新的肾脏领域，并推进肾脏研究 和全国范围内的发现。

Abstract The Physiology Core provides a series of graded technologies and expertise to investigators who wish to address fundamental questions about transport processes that occur in the tubular structures of the nephron and other relevant systems. To gain understanding of biological processes that mediate kidney function in health and disease states, in light of the phenotypic diversity and functional complexity of this organ, the use of strategies that allow specific nephron segments/cells/proteins to be studied in isolation under defined conditions is required. The Core, through its three Subcores, offers expertise and state-of-the-art technologies for studying the function and regulation of transport and other membrane resident proteins with progressive degrees of complexity from single molecules to model systems to native epithelia. The Single Tubule and Microperfusion Subcore provides tools and technologies to study the function and expression of transport proteins and associated regulatory pathways in single nephron segments isolated from small mammals and kidney organoids. The Electrophysiology Subcore provides expertise and a toolbox of electrophysiological approaches to study the function and regulation of transport proteins at the molecular and cellular levels in model systems and native epithelia. The Live-Cell Imaging Subcore offers imaging tools and technologies to assess ion transport, changes in membrane potential, cellular volume, intracellular mediators, and signaling molecules in immortalized cells and native preparations using chemical and genetically encoded fluorescent sensors. The Core will apply a multipronged approach and collaborate with the O’Brien Consortium to share specialized resources and technologies throughout the kidney research community, to lower the barrier to entry for early-stage investigators and researchers new to the kidney field, and to advance kidney research and discovery nationwide.