Mechanism of G Protein Beta5/ R7-RGS Protein/ R7BP Complex Signal Transduction

目前工作的目的是了解人类进化保守的GNB 5/R7-RGS/R7 BP复合物的信号功能，生物学和生理学。 为此，我们首先试图确定和表征的功能的Gbeta 5复合物及其组件在模型系统，如培养的神经元和/或神经内分泌细胞和细胞系，原代啮齿动物脑神经元，和基因修饰的小鼠。 Gnb 5和其他Gnb 5/R7-RGS/R7 BP复合物组分在小鼠中功能丧失的影响以及Gnb 5复合物在躯体感觉中的作用目前正在使用发育，行为，免疫组织化学和神经解剖学分析进行研究。从公开数据库收集的人类基因组数据的其他生物信息学研究正在进行，以更好地了解人类生殖系GNB 5突变的影响。

The purpose of the current work is to understand the signaling function, biology, and physiology of the evolutionarily-conserved GNB5/R7-RGS/R7BP complex in humans. To this end we are first trying to identify and characterize the functions of the Gbeta5 complex and its components in model systems such as cultured neuronal and/or neuroendocrine cells and cell lines, primary rodent brain neurons, and genetically-modified mice. The effect of the loss-of-function of Gnb5 and other Gnb5/R7-RGS/R7BP complex components in mice as well as the role of the Gnb5 complex in somatosensation are currently being examined using developmental, behavioral, immunohistochemical, and neuroanatomical analyses. Additional bioinformatic studies of human genomic data, gathered from publicly-available databases, are being performed to better understand the implications of germline GNB5 mutation in humans.