REGULATION OF PREMEIOTIC DNA REPLICATION IN S POMBE

描述（改编自研究者摘要）：长期目的 这项研究的目的是确定真核细胞如何调节这一过程 并将其与减数分裂中的其他事件偶联。 不能正确地调节减数分裂可导致染色体的不适当分离。 遗传物质，以及无法存活或受损的配子，这可能导致出生 缺陷 这项工作利用了一种简单的真核生物的遗传学， 裂殖酵母裂殖酵母 从以下方面获得的见解 从简单的遗传系统研究细胞现象有助于 描述发生在复杂生物体中的事件，包括人类， 因为减数分裂的过程是所有有性发育的 在真核生物中，很有可能许多元素会被保存下来。 该提案的具体目标如下。 第一，程度 有丝分裂和减数分裂DNA复制调控之间的重叠将是 评估。 已知营养细胞S期所需的基因，或 将复制与其他细胞周期事件偶联， 它们在减数分裂中的作用 减数分裂细胞对DNA损伤的反应将是 确定，并要求确定复制起点， 将测定减数分裂前DNA合成。 这将提供部分 减数分裂S期事件的描述及其分析工具 进一步. 第二，特异性影响减数分裂前DNA的新基因 复制，或减数分裂前DNA复制与下游DNA复制的偶联。 减数分裂事件将被分离。 最后，分子、生物化学和 将对鉴定的新基因座进行遗传分析， 交易行为调节器的网络，保持事件的顺序， 减数分裂 这将使我们能够全面地描述 真核生物模型中减数分裂前DNA复制。

DESCRIPTION (adapted from investigator's abstract): The long term objective of this research is to determine how eukaryotic cells regulate the process of premeiotic DNA replication and couple it to other events in meiosis. Failure to properly regulate meiosis can lead to improper segregation of the genetic material, and inviable or damaged gametes, which can result in birth defects. This work exploits the genetics of a simple eukaryotic organism, the fission yeast, Schizosaccharomyces pombe. The insights gained from studying cellular phenomena from simple genetic systems are helpful in describing the events occurring in complex organisms, including man, and because the process of meiosis is common to all sexually developing eukaryotes, there is a good chance that many elements will be conserved. The specific aims of the proposal are as follows. First, the degree of overlap between regulation of mitotic and meiotic DNA replication will be assessed. Genes known to be required for S phase in vegetative cells, or for coupling replication to other cell cycle events, will be analyzed for their role in meiosis. The response of meiotic cells to DNA damage will be determined, and the requirement for identified origins of replication in premeiotic DNA synthesis will be assayed. This will provide a partial description of the events of meiotic S phase as well as tools to analyze it further. Second, new genes specifically affecting premeiotic DNA replication, or the coupling of premeiotic DNA replication to downstream meiotic events will be isolated. Finally, molecular, biochemical, and genetic analysis will be carried out on the new loci identified to establish the network of trans-acting regulators that preserves the order of events in meiosis. This will allow a thorough description of the regulation of premeiotic DNA replication in a model eukaryote.